Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device.
Journal
Nature biomedical engineering
ISSN: 2157-846X
Titre abrégé: Nat Biomed Eng
Pays: England
ID NLM: 101696896
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
17
07
2020
accepted:
01
11
2020
pubmed:
5
12
2020
medline:
5
12
2020
entrez:
4
12
2020
Statut:
ppublish
Résumé
The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT-qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1-2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT-qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.
Identifiants
pubmed: 33273713
doi: 10.1038/s41551-020-00654-0
pii: 10.1038/s41551-020-00654-0
pmc: PMC8202505
mid: NIHMS1710821
doi:
Types de publication
Journal Article
Research Support, U.S. Gov't, P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1159-1167Subventions
Organisme : Department of Defense
ID : DOD-W81XWH1910194
Pays : International
Organisme : NCI NIH HHS
ID : R01 CA229777
Pays : United States
Organisme : Institute for Basic Science (IBS)
ID : IBS-R026-D1
Pays : International
Organisme : Department of Defense
ID : DOD-W81XWH1910199
Pays : International
Organisme : NIDA NIH HHS
ID : R21 DA049577
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA233360
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : ErratumIn
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