Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting.


Journal

Current protocols in chemical biology
ISSN: 2160-4762
Titre abrégé: Curr Protoc Chem Biol
Pays: United States
ID NLM: 101559235

Informations de publication

Date de publication:
12 2020
Historique:
entrez: 4 12 2020
pubmed: 5 12 2020
medline: 22 6 2021
Statut: ppublish

Résumé

Cytidine acetyltransferases are an emerging class of nucleic-acid-modifying enzymes responsible for the establishment of N

Identifiants

pubmed: 33275333
doi: 10.1002/cpch.89
doi:

Substances chimiques

Cytidine 5CSZ8459RP
RNA 63231-63-0
Acetyltransferases EC 2.3.1.-

Types de publication

Journal Article Research Support, N.I.H., Intramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

e89

Subventions

Organisme : Intramural NIH HHS
ID : ZIA BC011488
Pays : United States

Informations de copyright

© 2020 Wiley Periodicals LLC.

Références

Arango, D., Sturgill, D., & Oberdoerffer, S. (2019). Immunoprecipitation and sequencing of acetylated RNA. Bio-Protocol, 9(12), e3278. doi: 10.21769/BioProtoc.3278.
Balmus, G., Larrieu, D., Barros, A. C., Collins, C., Abrudan, M., Demir, M., … Jackson, S. P. (2018). Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome. Nature Communications, 9(1), 1700. doi: 10.1038/s41467-018-03770-3.
Guan, K.-L., Yu, W., Lin, Y., Xiong, Y., & Zhao, S. (2010). Generation of acetyllysine antibodies and affinity enrichment of acetylated peptides. Nature Protocols, 5(9), 1583-1595. doi: 10.1038/nprot.2010.117.
Ikeuchi, Y., Kitahara, K., & Suzuki, T. (2008). The RNA acetyltransferase driven by ATP hydrolysis synthesizes N4-acetylcytidine of tRNA anticodon. The EMBO Journal, 27(16), 2194-2203. doi: 10.1038/emboj.2008.154.
Jin, G., Xu, M., Zou, M., & Duan, S. (2020). The processing, gene regulation, biological functions, and clinical relevance of N4-acetylcytidine on RNA: A systematic review. Molecular Therapy. Nucleic Acids, 20, 13-24. doi: 10.1016/j.omtn.2020.01.037.
Johansson, M. J. O. (2004). The Saccharomyces cerevisiae TAN1 gene is required for N4-acetylcytidine formation in tRNA. RNA, 10(4), 712-719. doi: 10.1261/rna.5198204.
Larrieu, D., Britton, S., Demir, M., Rodriguez, R., & Jackson, S. P. (2014). Chemical inhibition of NAT10 corrects defects of laminopathic cells. Science, 344(6183), 527-532. doi: 10.1126/science.1252651.
Li, Q., Liu, X., Jin, K., Lu, M., Zhang, C., Du, X., & Xing, B. (2017). NAT10 is upregulated in hepatocellular carcinoma and enhances mutant p53 activity. BMC Cancer, 17(1), 605. doi: 10.1186/s12885-017-3570-4.
Mishima, E., & Abe, T. (2019). Immuno-northern blotting: Detection of modified RNA using gel separation and antibodies to modified nucleosides. Methods in Molecular Biology, 1870, 179-187. doi: 10.1007/978-1-4939-8808-2_13.
Mishima, E., Jinno, D., Akiyama, Y., Itoh, K., Nankumo, S., Shima, H., … Abe, T. (2015). Immuno-northern blotting: Detection of RNA modifications by using antibodies against modified nucleosides. PloS One, 10(11), e0143756. doi: 10.1371/journal.pone.0143756.
Orita, I., Futatsuishi, R., Adachi, K., Ohira, T., Kaneko, A., Minowa, K., … Fukui, T. (2019). Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance. Nucleic Acids Research, 47(4), 1964-1976. doi: 10.1093/nar/gky1313.
Sas-Chen, A., Thomas, J. M., Matzov, D., Taoka, M., Nance, K. D., Nir, R., … Schwartz, S. (2020). Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping. Nature, 583, 638-643. doi: 10.1038/s41586-020-2418-2.
Sharma, S., Langhendries, J.-L., Watzinger, P., Kötter, P., Entian, K.-D., & Lafontaine, D. L. J. (2015). Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1. Nucleic Acids Research, 43(4), 2242-2258.doi: 10.1093/nar/gkv075.
Sharma, S., Yang, J., van Nues, R., Watzinger, P., Kötter, P., Lafontaine, D. L. J., … Entian, K.-D. (2017). Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation. PLoS Genetics, 13(5), e1006804. doi: 10.1371/journal.pgen.1006804.
Sinclair, W. R., Arango, D., Shrimp, J. H., Zengeya, T. T., Thomas, J. M., Montgomery, D. C., … Meier, J. L. (2017). Profiling cytidine acetylation with specific affinity and reactivity. ACS Chemical Biology, 12(12), 2922-2926. doi: 10.1021/acschembio.7b00734.
Taniguchi, T., Miyauchi, K., Sakaguchi, Y., Yamashita, S., Soma, A., Tomita, K., & Suzuki, T. (2018). Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis. Nature Chemical Biology, 14(11), 1010-1020. doi: 10.1038/s41589-018-0119-z.
Thomas, J. M., Briney, C. A., Nance, K. D., Lopez, J. E., Thorpe, A. L., Fox, S. D., … Meier, J. L. (2018). A chemical signature for cytidine acetylation in RNA. Journal of the American Chemical Society, 140(40), 12667-12670. doi: 10.1021/jacs.8b06636.
Thomas, J. M., Bryson, K. M., & Meier, J. L. (2019). Nucleotide resolution sequencing of N4-acetylcytidine in RNA. Methods in Enzymology, 621, 31-51. doi: 10.1016/bs.mie.2019.02.022.
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Auteurs

Keri M Bryson (KM)

Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.

Supuni Thalalla-Gamage (S)

Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.

Jordan L Meier (JL)

Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.

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