Comparison of the PU.1 transcriptional regulome and interactome in human and mouse inflammatory dendritic cells.
PU.1
dendritic cells
interactome
interspecies
transcription factor
transcriptome
Journal
Journal of leukocyte biology
ISSN: 1938-3673
Titre abrégé: J Leukoc Biol
Pays: England
ID NLM: 8405628
Informations de publication
Date de publication:
02 Dec 2020
02 Dec 2020
Historique:
received:
11
12
2019
revised:
18
11
2020
accepted:
18
11
2020
entrez:
8
12
2020
pubmed:
9
12
2020
medline:
9
12
2020
Statut:
aheadofprint
Résumé
Dendritic cells (DCs) are key immune modulators and are able to mount immune responses or tolerance. DC differentiation and activation imply a plethora of molecular and cellular responses, including transcriptional changes. PU.1 is a highly expressed transcription factor in DCs and coordinates relevant aspects of DC biology. Due to their role as immune regulators, DCs pose as a promising immunotherapy tool. However, some of their functional features, such as survival, activation, or migration, are compromised due to the limitations to simulate in vitro the physiologic DC differentiation process. A better knowledge of transcriptional programs would allow the identification of potential targets for manipulation with the aim of obtaining "qualified" DCs for immunotherapy purposes. Most of the current knowledge regarding DC biology derives from studies using mouse models, which not always find a parallel in human. In the present study, we dissect the PU.1 transcriptional regulome and interactome in mouse and human DCs, in the steady state or LPS activated. The PU.1 transcriptional regulome was identified by performing PU.1 chromatin immunoprecipitation followed by high-throughput sequencing and pairing these data with RNAsequencing data. The PU.1 interactome was identified by performing PU.1 immunoprecipitation followed by mass spectrometry analysis. Our results portray PU.1 as a pivotal factor that plays an important role in the regulation of genes required for proper DC activation and function, and assures the repression of nonlineage genes. The interspecies differences between human and mouse DCs are surprisingly substantial, highlighting the need to study the biology of human DCs.
Identifiants
pubmed: 33289106
doi: 10.1002/JLB.6A1219-711RRR
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Netherlands Scientific Organization
ID : VENI 863.09.012
Organisme : Spanish Ministerio de Economía y Competitividad
ID : RYC-2013-12587
Organisme : Co nsejería de Ciencia, Innovación y Universidad del Principado de Asturias (Spain)
ID : PA-20-PF-BP19-014
Organisme : Fundación para la Investigación y la Innovación Biosanitaria de Asturias (FINBA, Spain)
Informations de copyright
©2020 Society for Leukocyte Biology.
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