Thickness and Curvature Changes of Human Corneal Grafts in Dextran-Containing Organ Culture Medium Before Keratoplasty.
Cornea
/ drug effects
Corneal Edema
/ diagnostic imaging
Culture Media
Dextrans
/ therapeutic use
Female
Humans
Keratoplasty, Penetrating
Male
Organ Culture Techniques
Organ Preservation Solutions
Organ Size
Plasma Substitutes
/ therapeutic use
Time Factors
Tissue Donors
Tomography, Optical Coherence
Journal
Cornea
ISSN: 1536-4798
Titre abrégé: Cornea
Pays: United States
ID NLM: 8216186
Informations de publication
Date de publication:
01 Jun 2021
01 Jun 2021
Historique:
received:
17
05
2020
accepted:
07
08
2020
pubmed:
9
12
2020
medline:
6
11
2021
entrez:
8
12
2020
Statut:
ppublish
Résumé
To determine the changes of corneal thickness and curvature of human corneal grafts in organ culture medium II, containing dextran T500 6%, before keratoplasty. We examined the tomography of 24 corneas from our eye bank transferred from medium I into medium II. Images were repeated hourly during 24 hours using an anterior segment optical coherence tomography. The central corneal thickness (CCT) was measured with the manual measurement tool of the anterior segment optical coherence tomography. The radii of curvature (anterior flat and steep and posterior flat and steep) were measured with a MATLAB self-programmed software for "sterile donor tomography." The mean CCT (±SD) at baseline (T0) was 727 ± 156 μm. It reached 581 ± 103, 506 ± 84, 472 ± 79, and 456±7 μm after 6, 12, 18, and 24 hours, respectively. After 12 hours, 83% of the final deswelling was achieved. The radii of curvature (±SD) at baseline (T0) were (posterior flat, posterior steep, anterior flat, and anterior steep) 6.6 ± 0.5, 6.2 ± 0.5, 7.7 ± 0.4, and 7.4 ± 0.4 mm, respectively. After 24 hours, the radii of curvature reached 6.8 ± 0.1, 6.6 ± 0.3, 7.6 ± 0.1, and 7.4 ± 0.2 mm, respectively. The kinetics of the deswelling process in medium II follow a hyperbolic curve. Considering a CCT of 506 μm at T12, we assume that a time interval of 12 hours in medium II might be enough for clinical purposes. This result might help to keep storage in medium II as short as possible to escape potential toxic effects of dextran in medium II. The radius of curvature does not seem to change within 24 hours for all measured surfaces.
Identifiants
pubmed: 33290320
pii: 00003226-202106000-00011
doi: 10.1097/ICO.0000000000002543
doi:
Substances chimiques
Culture Media
0
Dextrans
0
Organ Preservation Solutions
0
Plasma Substitutes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
733-740Informations de copyright
Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
The authors have no funding or conflicts of interest to disclose.
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