Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR).
Australian wildlife
Brushtail possum
High-resolution melting PCR
Marsupials
SYTO 9
Trypanosomes
Woylie
Journal
International journal for parasitology. Parasites and wildlife
ISSN: 2213-2244
Titre abrégé: Int J Parasitol Parasites Wildl
Pays: England
ID NLM: 101599824
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
31
08
2020
revised:
30
09
2020
accepted:
11
11
2020
entrez:
9
12
2020
pubmed:
10
12
2020
medline:
10
12
2020
Statut:
epublish
Résumé
Conventional nested PCR and Sanger sequencing methods are currently the gold standards for detecting trypanosomes in wildlife. However, these techniques are time-consuming and can often overlook mixed infections. True trypanosome prevalence can thus be underrepresented. Here, we designed an 18S rDNA-based real-time quantitative PCR (qPCR) assay coupled with High-Resolution Melting Analysis (HRMA) to detect and discriminate three
Identifiants
pubmed: 33294365
doi: 10.1016/j.ijppaw.2020.11.003
pii: S2213-2244(20)30104-8
pmc: PMC7691731
doi:
Types de publication
Journal Article
Langues
eng
Pagination
261-268Informations de copyright
© 2020 The Authors.
Références
Parasit Vectors. 2014 Apr 07;7:169
pubmed: 24708757
Adv Parasitol. 2001;48:1-56
pubmed: 11013754
Int J Evol Biol. 2013;2013:571939
pubmed: 23401844
Parasitology. 2009 Sep;136(11):1269-79
pubmed: 19660159
Comp Immunol Microbiol Infect Dis. 2019 Dec;67:101364
pubmed: 31590033
Int J Parasitol Parasites Wildl. 2014 Mar 15;3(2):57-66
pubmed: 25161902
Anal Biochem. 1983 Jul 1;132(1):14-9
pubmed: 6312834
Protist. 2015 Nov;166(5):551-68
pubmed: 26466163
BMC Genet. 2009 Feb 17;10:5
pubmed: 19222838
Int J Parasitol Parasites Wildl. 2013 Mar 29;2:77-89
pubmed: 24533319
Int J Parasitol Parasites Wildl. 2018 Jun 15;7(2):221-227
pubmed: 29942738
Evolution. 1985 Jul;39(4):783-791
pubmed: 28561359
Mol Biol Evol. 1993 May;10(3):512-26
pubmed: 8336541
PLoS One. 2017 Jul 13;12(7):e0181279
pubmed: 28704541
Int J Parasitol Parasites Wildl. 2018 Jan 28;7(1):58-67
pubmed: 29988778
Exp Parasitol. 2015 Jun;153:105-10
pubmed: 25816975
Trends Parasitol. 2015 Oct;31(10):460-469
pubmed: 26433249
Avian Pathol. 2009 Feb;38(1):13-9
pubmed: 19156576
Folia Parasitol (Praha). 2014 Dec;61(6):495-504
pubmed: 25651690
Vet Parasitol. 2011 Dec 29;183(1-2):21-30
pubmed: 21802854
Exp Parasitol. 2013 Sep;135(1):142-7
pubmed: 23838581
Int J Parasitol. 1999 Feb;29(2):331-9
pubmed: 10221634
J Parasitol. 2011 Aug;97(4):725-30
pubmed: 21506835
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Parasit Vectors. 2019 Mar 21;12(1):126
pubmed: 30898141
Protist. 2016 Nov;167(5):425-439
pubmed: 27561173
Mem Inst Oswaldo Cruz. 2015 Dec;110(8):956-65
pubmed: 26602872
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
Parasit Vectors. 2018 Aug 2;11(1):447
pubmed: 30071897
Parasitology. 2019 Jan;146(1):1-27
pubmed: 29898792
J Clin Microbiol. 2002 Jul;40(7):2335-8
pubmed: 12089244