Pyroptotic cell death by exposure to 1-butanol in H9c2 cardiomyoblastoma cells.
1-Butanol
Biochemistry
Caspase-3
Cell biology
GSDME
H9c2 cells
Immunology
Pathophysiology
Pyroptosis
Toxicology
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
17
08
2020
revised:
03
10
2020
accepted:
10
11
2020
entrez:
9
12
2020
pubmed:
10
12
2020
medline:
10
12
2020
Statut:
epublish
Résumé
The aim of this study is to examine the molecular mechanism of cytotoxicity caused by direct exposure to short chain alcohol. We showed previously that exposing H9c2 cardiomyoblastoma cells to 150 mM 1-butanol results in cell death within 1 h through an intrinsic apoptotic pathway. The cell death is accompanied by plasma membrane blebbing and caspase-3 activation. Here we show that a higher concentration (200 mM) of 1-butanol, as well as prolonged exposure (3-6 h) to 150 mM 1-butanol, induces plasma membrane ballooning, a characteristic feature of pyroptosis. Although gasderminD (GSDMD) cleavage by caspase-1 was not observed, GSDME cleavage by caspase-3 was observed during exposure to 150 mM 1-butanol for 6 h. We conclude that pyroptotic cell death by 1-butanol in H9c2 cardiomyoblastoma cells should occur via the caspase-3-GSDME pathway, revealing that 1-butanol could induce not only apoptosis but also pyroptosis in the cells.
Identifiants
pubmed: 33294662
doi: 10.1016/j.heliyon.2020.e05503
pii: S2405-8440(20)32346-X
pmc: PMC7683309
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e05503Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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