Automatic imaging of Drosophila embryos with light sheet fluorescence microscopy on chip.
3D imaging
femtosecond laser micromachining
integrated optics
lab on a chip
light sheet fluorescence microscopy
microfluidics
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
02
10
2020
revised:
22
11
2020
accepted:
07
12
2020
pubmed:
10
12
2020
medline:
13
7
2021
entrez:
9
12
2020
Statut:
ppublish
Résumé
We present a microscope on chip for automated imaging of Drosophila embryos by light sheet fluorescence microscopy. This integrated device, constituted by both optical and microfluidic components, allows the automatic acquisition of a 3D stack of images for specimens diluted in a liquid suspension. The device has been fully optimized to address the challenges related to the specimens under investigation. Indeed, the thickness and the high ellipticity of Drosophila embryos can degrade the image quality. In this regard, optical and fluidic optimization has been carried out to implement dual-sided illumination and automatic sample orientation. In addition, we highlight the dual color investigation capabilities of this device, by processing two sample populations encoding different fluorescent proteins. This work was made possible by the versatility of the used fabrication technique, femtosecond laser micromachining, which allows straightforward fabrication of both optical and fluidic components in glass substrates.
Identifiants
pubmed: 33295053
doi: 10.1002/jbio.202000396
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000396Informations de copyright
© 2020 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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