Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.
Adenosine Diphosphate
/ metabolism
Adenosine Triphosphatases
/ metabolism
Animals
CHO Cells
Calcium
/ deficiency
Cricetulus
Crystallography, X-Ray
Drosophila
Endoplasmic Reticulum
/ metabolism
Endoplasmic Reticulum Chaperone BiP
Escherichia coli
Flow Cytometry
HSP70 Heat-Shock Proteins
/ metabolism
Heat-Shock Proteins
/ metabolism
Immunoprecipitation
Proteostasis
Unfolded Protein Response
BiP
Grp78
biochemistry
calcium
cell biology
chemical biology
endoplasmic reticulum
none
oligomerisation
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
09 12 2020
09 12 2020
Historique:
received:
30
08
2020
accepted:
08
12
2020
pubmed:
10
12
2020
medline:
19
3
2021
entrez:
9
12
2020
Statut:
epublish
Résumé
The metazoan endoplasmic reticulum (ER) serves both as a hub for maturation of secreted proteins and as an intracellular calcium storage compartment, facilitating calcium-release-dependent cellular processes. ER calcium depletion robustly activates the unfolded protein response (UPR). However, it is unclear how fluctuations in ER calcium impact organellar proteostasis. Here, we report that calcium selectively affects the dynamics of the abundant metazoan ER Hsp70 chaperone BiP, by enhancing its affinity for ADP. In the calcium-replete ER, ADP rebinding to post-ATP hydrolysis BiP-substrate complexes competes with ATP binding during both spontaneous and co-chaperone-assisted nucleotide exchange, favouring substrate retention. Conversely, in the calcium-depleted ER, relative acceleration of ADP-to-ATP exchange favours substrate release. These findings explain the rapid dissociation of certain substrates from BiP observed in the calcium-depleted ER and suggest a mechanism for tuning ER quality control and coupling UPR activity to signals that mobilise ER calcium in secretory cells.
Identifiants
pubmed: 33295873
doi: 10.7554/eLife.62601
pii: 62601
pmc: PMC7758071
doi:
pii:
Substances chimiques
Endoplasmic Reticulum Chaperone BiP
0
HSP70 Heat-Shock Proteins
0
Heat-Shock Proteins
0
Adenosine Diphosphate
61D2G4IYVH
Adenosine Triphosphatases
EC 3.6.1.-
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
ID : 200848/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome
ID : 996 100140
Pays : International
Organisme : Wellcome
ID : 200848/Z/16/Z
Pays : International
Informations de copyright
© 2020, Preissler et al.
Déclaration de conflit d'intérêts
SP, CR, YY, LP, AC No competing interests declared, DR Reviewing editor, eLife
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