S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue.
Journal
NPJ breast cancer
ISSN: 2374-4677
Titre abrégé: NPJ Breast Cancer
Pays: United States
ID NLM: 101674891
Informations de publication
Date de publication:
24 Nov 2020
24 Nov 2020
Historique:
received:
12
11
2019
accepted:
21
10
2020
entrez:
10
12
2020
pubmed:
11
12
2020
medline:
11
12
2020
Statut:
epublish
Résumé
Immunohistochemical (IHC) staining in breast cancer shows both gain and loss of COX2 expression with disease risk and progression. We investigated four common COX2 antibody clones and found high specificity for purified human COX2 for three clones; however, recognition of COX2 in cell lysates was clone dependent. Biochemical characterization revealed two distinct forms of COX2, with SP21 recognizing an S-nitrosylated form, and CX229 and CX294 recognizing non-nitrosylated COX2 antigen. We found S-nitrosylated and non-nitrosylated COX2 occupy different subcellular locations in normal and breast cancer tissue, implicating distinct synthetic/trafficking pathways and function. Dual stains of ~2000 breast cancer cases show early-onset breast cancer had increased expression of both forms of COX2 compared to postmenopausal cases. Our results highlight the strengths of using multiple, highly characterized antibody clones for COX2 IHC studies and raise the prospect that S-nitrosylation of COX2 may play a role in breast cancer biology.
Identifiants
pubmed: 33298921
doi: 10.1038/s41523-020-00204-6
pii: 10.1038/s41523-020-00204-6
pmc: PMC7686348
doi:
Types de publication
Journal Article
Langues
eng
Pagination
62Subventions
Organisme : NCI NIH HHS
ID : R01 CA169175
Pays : United States
Organisme : NCI NIH HHS
ID : UM1 CA186107
Pays : United States
Organisme : Division of Cancer Prevention, National Cancer Institute (NCI Division of Cancer Prevention)
ID : T32CA009001
Organisme : Division of Cancer Prevention, National Cancer Institute (NCI Division of Cancer Prevention)
ID : R01CA160246
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