Low-intensity vibration restores nuclear YAP levels and acute YAP nuclear shuttling in mesenchymal stem cells subjected to simulated microgravity.
Journal
NPJ microgravity
ISSN: 2373-8065
Titre abrégé: NPJ Microgravity
Pays: United States
ID NLM: 101703605
Informations de publication
Date de publication:
01 Dec 2020
01 Dec 2020
Historique:
received:
26
05
2020
accepted:
08
10
2020
entrez:
10
12
2020
pubmed:
11
12
2020
medline:
11
12
2020
Statut:
epublish
Résumé
Reducing the musculoskeletal deterioration that astronauts experience in microgravity requires countermeasures that can improve the effectiveness of otherwise rigorous and time-expensive exercise regimens in space. The ability of low-intensity vibrations (LIV) to activate force-responsive signaling pathways in cells suggests LIV as a potential countermeasure to improve cell responsiveness to subsequent mechanical challenge. Mechanoresponse of mesenchymal stem cells (MSC), which maintain bone-making osteoblasts, is in part controlled by the "mechanotransducer" protein YAP (Yes-associated protein), which is shuttled into the nucleus in response to cyto-mechanical forces. Here, using YAP nuclear shuttling as a measurement outcome, we tested the effect of 72 h of clinostat-induced simulated microgravity (SMG) and daily LIV application (LIV
Identifiants
pubmed: 33298964
doi: 10.1038/s41526-020-00125-5
pii: 10.1038/s41526-020-00125-5
pmc: PMC7708987
doi:
Types de publication
Journal Article
Langues
eng
Pagination
35Subventions
Organisme : U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging)
ID : R01AG059923
Organisme : National Science Foundation (NSF)
ID : 2025505
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : 5P2CHD086843-03
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)
ID : P20GM109095
Organisme : NIGMS NIH HHS
ID : P20 GM103408
Pays : United States
Organisme : Idaho Space Grant Consortium (NASA Idaho Space Grant Consortium)
ID : NNX15AI04H
Organisme : NIGMS NIH HHS
ID : P20 GM109095
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)
ID : P20GM103408
Organisme : NIA NIH HHS
ID : R01 AG059923
Pays : United States
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