Phage lysin that specifically eliminates Clostridium botulinum Group I cells.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
09 12 2020
Historique:
received: 04 06 2020
accepted: 23 11 2020
entrez: 10 12 2020
pubmed: 11 12 2020
medline: 1 5 2021
Statut: epublish

Résumé

Clostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth and causing life-threatening neuroparalysis, botulism. While high temperature can be utilized to eliminate C. botulinum spores and the neurotoxin, non-thermal elimination of newly germinated C. botulinum cells before onset of toxin production could provide an alternative or additional factor controlling the risk of botulism in some applications. Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the concentration of 5 µM, but shows little or no lytic activity against C. botulinum Group II or III or other Firmicutes strains. CBO1751 is active at pH from 6.5 to 10.5. The lytic activity of CBO1751 is tolerant to NaCl (200 mM), but highly susceptible to divalent cations Ca

Identifiants

pubmed: 33299101
doi: 10.1038/s41598-020-78622-6
pii: 10.1038/s41598-020-78622-6
pmc: PMC7725837
doi:

Substances chimiques

Enzymes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

21571

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Auteurs

Zhen Zhang (Z)

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P. O. Box 66, 00014, Helsinki, Finland.

Meeri Lahti (M)

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P. O. Box 66, 00014, Helsinki, Finland.

François P Douillard (FP)

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P. O. Box 66, 00014, Helsinki, Finland.

Hannu Korkeala (H)

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P. O. Box 66, 00014, Helsinki, Finland.

Miia Lindström (M)

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P. O. Box 66, 00014, Helsinki, Finland. miia.lindstrom@helsinki.fi.

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Classifications MeSH