Protracted viral shedding and viral load are associated with ICU mortality in Covid-19 patients with acute respiratory failure.

Acute respiratory distress syndrome Acute respiratory failure COVID-19 Polymerase chain reaction SARS-COV-2 Viral load Viral shedding

Journal

Annals of intensive care
ISSN: 2110-5820
Titre abrégé: Ann Intensive Care
Pays: Germany
ID NLM: 101562873

Informations de publication

Date de publication:
10 Dec 2020
Historique:
received: 03 10 2020
accepted: 25 11 2020
entrez: 10 12 2020
pubmed: 11 12 2020
medline: 11 12 2020
Statut: epublish

Résumé

Protracted viral shedding is common in hospitalized patients with COVID-19 pneumonia, and up to 40% display signs of pulmonary fibrosis on computed tomography (CT) after hospital discharge. We hypothesized that COVID-19 patients with acute respiratory failure (ARF) who die in intensive care units (ICU) have a lower viral clearance in the respiratory tract than ICU patients discharged alive, and that protracted viral shedding in respiratory samples is associated with patterns of fibroproliferation on lung CT. We, therefore, conducted a retrospective observational study, in 2 ICU of Lyon university hospital. 129 patients were included in the study, of whom 44 (34%) died in ICU. 432 RT-PCR for SARS-CoV-2 were performed and 137 CT scans were analyzed. Viral load was significantly higher in patients deceased as compared to patients alive at ICU discharge (p < 0.001), after adjustment for the site of viral sampling and RT-PCR technique. The median time to SARS-CoV-2 negativation on RT-PCR was 19 days [CI Viral load in respiratory samples is significantly lower and viral shedding significantly shorter in ICU survivors of COVID-19 associated acute respiratory failure. Protracted viral shedding is unrelated to occurrence of fibrosis on lung CT.

Sections du résumé

BACKGROUND BACKGROUND
Protracted viral shedding is common in hospitalized patients with COVID-19 pneumonia, and up to 40% display signs of pulmonary fibrosis on computed tomography (CT) after hospital discharge. We hypothesized that COVID-19 patients with acute respiratory failure (ARF) who die in intensive care units (ICU) have a lower viral clearance in the respiratory tract than ICU patients discharged alive, and that protracted viral shedding in respiratory samples is associated with patterns of fibroproliferation on lung CT. We, therefore, conducted a retrospective observational study, in 2 ICU of Lyon university hospital.
RESULTS RESULTS
129 patients were included in the study, of whom 44 (34%) died in ICU. 432 RT-PCR for SARS-CoV-2 were performed and 137 CT scans were analyzed. Viral load was significantly higher in patients deceased as compared to patients alive at ICU discharge (p < 0.001), after adjustment for the site of viral sampling and RT-PCR technique. The median time to SARS-CoV-2 negativation on RT-PCR was 19 days [CI
CONCLUSIONS CONCLUSIONS
Viral load in respiratory samples is significantly lower and viral shedding significantly shorter in ICU survivors of COVID-19 associated acute respiratory failure. Protracted viral shedding is unrelated to occurrence of fibrosis on lung CT.

Identifiants

pubmed: 33301059
doi: 10.1186/s13613-020-00783-4
pii: 10.1186/s13613-020-00783-4
pmc: PMC7725883
doi:

Types de publication

Journal Article

Langues

eng

Pagination

167

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Auteurs

L Bitker (L)

Service de Médecine Intensive Réanimation, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France.
Université de Lyon, Université Claude Bernard, Lyon 1, INSA-Lyon, UJM-Saint Etienne, CNRS, Inserm, CREATIS, UMR 5220, U1206, 69621, Lyon, France.

F Dhelft (F)

Service de Médecine Intensive Réanimation, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France.
Université de Lyon, Université Claude Bernard, Lyon 1, INSA-Lyon, UJM-Saint Etienne, CNRS, Inserm, CREATIS, UMR 5220, U1206, 69621, Lyon, France.

L Chauvelot (L)

Service de Médecine Intensive Réanimation, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France.

E Frobert (E)

CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Team Virpath, 69007, Lyon, France.
Laboratoire de Virologie, Institut des Agents Infectieux, Hospices Civils de Lyon, National Reference Center for Respiratory Viruses, Department of Virology, Infective Agents Institute, North Hospital Network, Lyon, France; Virpath Laboratory, International Center of Research in Infectiology, INSERM U1111, CNRS-UMR 5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon, Université Claude Bernard, Lyon, France.
Université de Lyon, Université Claude Bernard, Lyon 1, Lyon, France.

L Folliet (L)

Service de Médecine Intensive Réanimation, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France.

M Mezidi (M)

Service de Médecine Intensive Réanimation, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France.
Université de Lyon, Université Claude Bernard, Lyon 1, Lyon, France.

S Trouillet-Assant (S)

CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Team Virpath, 69007, Lyon, France.
Joint Research Unit Hospices Civils de Lyon-bioMérieux, Hospices Civils de Lyon, Lyon Sud Hospital, Pierre-Bénite, France.

A Belot (A)

National Referee Centre for Rheumatic AutoImmune and Systemic Diseases in childrEn (RAISE), Lyon, France.
Pediatric Nephrology, Rheumatology, Dermatology Unit, Hospices Civils de Lyon, Pierre-Bénite, France.

B Lina (B)

CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Team Virpath, 69007, Lyon, France.
Laboratoire de Virologie, Institut des Agents Infectieux, Hospices Civils de Lyon, National Reference Center for Respiratory Viruses, Department of Virology, Infective Agents Institute, North Hospital Network, Lyon, France; Virpath Laboratory, International Center of Research in Infectiology, INSERM U1111, CNRS-UMR 5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon, Université Claude Bernard, Lyon, France.
Université de Lyon, Université Claude Bernard, Lyon 1, Lyon, France.

F Wallet (F)

Service de Réanimation Polyvalente, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, Pierre-Bénite, France.

J C Richard (JC)

Service de Médecine Intensive Réanimation, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France. j-christophe.richard@chu-lyon.fr.
Université de Lyon, Université Claude Bernard, Lyon 1, INSA-Lyon, UJM-Saint Etienne, CNRS, Inserm, CREATIS, UMR 5220, U1206, 69621, Lyon, France. j-christophe.richard@chu-lyon.fr.

Classifications MeSH