switchSENSE Technology for Analysis of DNA Polymerase Kinetics.
Biosensor
DNA polymerase
HIV reverse transcriptase
Kinetics
switchSENSE technology
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
10
12
2020
pubmed:
11
12
2020
medline:
1
4
2021
Statut:
ppublish
Résumé
The switchSENSE technology is a recent approach based on surface sensor chips for the analysis of interactions of macromolecules. The technology relies on electro-switchable DNA nanolevers tethered at one end on a gold surface via a sulfur linker and labeled with a Cy3 dye on the other end. The switchSENSE approach is effective in the determination of a large panel of biophysical parameters such as binding kinetics, dissociation constant, hydrodynamic radius, or melting temperature. In addition, it can also give access to some enzymatic data such as nuclease or polymerase activity. Here we describe a DNA polymerase assay that allows retrieving, in a single experimental set, association and dissociation rates, as well as the catalytic rate of the enzyme.
Identifiants
pubmed: 33301116
doi: 10.1007/978-1-0716-1126-5_8
doi:
Substances chimiques
HIV Reverse Transcriptase
EC 2.7.7.49
DNA-Directed DNA Polymerase
EC 2.7.7.7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-153Références
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