Alteration in the Culex pipiens transcriptome reveals diverse mechanisms of the mosquito immune system implicated upon Rift Valley fever phlebovirus exposure.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
29
01
2020
accepted:
10
10
2020
revised:
22
12
2020
pubmed:
11
12
2020
medline:
23
2
2021
entrez:
10
12
2020
Statut:
epublish
Résumé
Rift Valley fever phlebovirus (RVFV) causes an emerging zoonotic disease and is mainly transmitted by Culex and Aedes mosquitoes. While Aedes aegypti-dengue virus (DENV) is the most studied model, less is known about the genes involved in infection-responses in other mosquito-arboviruses pairing. The main objective was to investigate the molecular responses of Cx. pipiens to RVFV exposure focusing mainly on genes implicated in innate immune responses. Mosquitoes were fed with blood spiked with RVFV. The fully-engorged females were pooled at 3 different time points: 2 hours post-exposure (hpe), 3- and 14-days post-exposure (dpe). Pools of mosquitoes fed with non-infected blood were also collected for comparisons. Total RNA from each mosquito pool was subjected to RNA-seq analysis and a de novo transcriptome was constructed. A total of 451 differentially expressed genes (DEG) were identified. Most of the transcriptomic alterations were found at an early infection stage after RVFV exposure. Forty-eight DEG related to immune infection-response were characterized. Most of them were related with the RNAi system, Toll and IMD pathways, ubiquitination pathway and apoptosis. Our findings provide for the first time a comprehensive view on Cx. pipiens-RVFV interactions at the molecular level. The early depletion of RNAi pathway genes at the onset of the RVFV infection would allow viral replication in mosquitoes. While genes from the Toll and IMD immune pathways were altered in response to RVFV none of the DEG were related to the JAK/STAT pathway. The fact that most of the DEG involved in the Ubiquitin-proteasome pathway (UPP) or apoptosis were found at an early stage of infection would suggest that apoptosis plays a regulatory role in infected Cx. pipiens midguts. This study provides a number of target genes that could be used to identify new molecular targets for vector control.
Identifiants
pubmed: 33301456
doi: 10.1371/journal.pntd.0008870
pii: PNTD-D-20-00154
pmc: PMC7755283
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0008870Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Virology. 2009 Mar 1;385(1):47-57
pubmed: 19068261
Proc Natl Acad Sci U S A. 2014 May 13;111(19):7108-13
pubmed: 24778219
Viruses. 2014 Nov 19;6(11):4479-504
pubmed: 25415198
PLoS Negl Trop Dis. 2014 Jul 24;8(7):e2994
pubmed: 25058001
Jundishapur J Microbiol. 2015 Jul 25;8(7):e24757
pubmed: 26421138
J Med Entomol. 1998 Nov;35(6):918-21
pubmed: 9835680
PLoS Negl Trop Dis. 2016 Mar 03;10(3):e0004543
pubmed: 26938868
J Insect Physiol. 2014 Oct;69:80-8
pubmed: 24842780
PLoS One. 2013 Oct 11;8(10):e76535
pubmed: 24146884
Insect Biochem Mol Biol. 2005 Nov;35(11):1293-307
pubmed: 16203210
Curr Opin Virol. 2015 Dec;15:119-26
pubmed: 26629932
PLoS Negl Trop Dis. 2015 Jul 01;9(7):e0003885
pubmed: 26132143
Annu Rev Immunol. 2007;25:697-743
pubmed: 17201680
mSphere. 2017 Nov 22;2(6):
pubmed: 29202041
Nat Biotechnol. 2013 Jan;31(1):46-53
pubmed: 23222703
Nat Immunol. 2008 Dec;9(12):1425-32
pubmed: 18953338
Cell Death Differ. 2010 Jan;17(1):61-7
pubmed: 19498442
Adv Exp Med Biol. 2013;768:127-45
pubmed: 23224968
Dev Comp Immunol. 2010 Jun;34(6):625-9
pubmed: 20079370
Bioinformatics. 2010 Feb 15;26(4):493-500
pubmed: 20022975
PLoS One. 2012;7(1):e30861
pubmed: 22292064
J S Afr Vet Assoc. 1977 Mar;48(1):45-8
pubmed: 874947
J Innate Immun. 2016;8(2):199-210
pubmed: 26694862
Nat Biotechnol. 2011 May 15;29(7):644-52
pubmed: 21572440
PLoS One. 2013 Oct 21;8(10):e78077
pubmed: 24205097
PLoS Negl Trop Dis. 2013 Jul 04;7(7):e2295
pubmed: 23861987
Viruses. 2013 Aug 16;5(8):2019-42
pubmed: 23959328
Science. 2007 Jun 22;316(5832):1738-43
pubmed: 17588928
Cell. 2015 Nov 19;163(5):1108-1123
pubmed: 26582131
PLoS Pathog. 2009 Jul;5(7):e1000502
pubmed: 19578437
PLoS Negl Trop Dis. 2014 Apr 24;8(4):e2823
pubmed: 24762775
Genome Biol. 2014;15(12):550
pubmed: 25516281
Sci Rep. 2017 Oct 30;7(1):14385
pubmed: 29085037
Am J Trop Med Hyg. 2007 Jan;76(1):118-28
pubmed: 17255239
Dev Comp Immunol. 2018 Jun;83:70-79
pubmed: 29198776
Med Vet Entomol. 2017 Dec;31(4):365-372
pubmed: 28782121
Nucleic Acids Res. 2008 Jun;36(10):3420-35
pubmed: 18445632
Apoptosis. 2006 Sep;11(9):1643-51
pubmed: 16820968
Nat Methods. 2011 Sep 29;8(10):785-6
pubmed: 21959131
PLoS One. 2012;7(10):e47350
pubmed: 23077596
Nucleus. 2010 Jul-Aug;1(4):332-6
pubmed: 21327081
Annu Rev Entomol. 2016;61:395-415
pubmed: 26982443
Trends Cell Biol. 2002 May;12(5):216-21
pubmed: 12062168
Nature. 2014 Jun 12;510(7504):288-92
pubmed: 24870241
J Gen Virol. 2013 Jul;94(Pt 7):1680-1689
pubmed: 23559478
Bioinformatics. 2015 Oct 1;31(19):3210-2
pubmed: 26059717
Sci Signal. 2010 Oct 26;3(145):re8
pubmed: 20978240
Vector Borne Zoonotic Dis. 2008 Dec;8(6):749-53
pubmed: 18620510
Viruses. 2019 Jun 04;11(6):
pubmed: 31167461
Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18915-20
pubmed: 23027947
Nucleic Acids Res. 2017 Jan 4;45(D1):D158-D169
pubmed: 27899622
Nucleic Acids Res. 2015 Apr 20;43(7):3688-700
pubmed: 25765650
Insect Biochem Mol Biol. 2009 May-Jun;39(5-6):395-402
pubmed: 19366631
PLoS Negl Trop Dis. 2017 Jan 6;11(1):e0005272
pubmed: 28060823
PLoS One. 2011;6(7):e21800
pubmed: 21789182
Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15862-7
pubmed: 20798052
PLoS Pathog. 2009 Feb;5(2):e1000299
pubmed: 19214215
Am J Trop Med Hyg. 2005 May;72(5):631-7
pubmed: 15891140
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Parasit Vectors. 2019 Apr 11;12(1):165
pubmed: 30975197
Front Microbiol. 2017 Oct 23;8:2050
pubmed: 29109710
Cell Death Dis. 2015 Dec 17;6:e2018
pubmed: 26673663
Vaccine. 2015 Oct 13;33(42):5520-5531
pubmed: 26296499
Environ Microbiol. 2013 Nov;15(11):2966-79
pubmed: 23809263
Emerg Microbes Infect. 2019;8(1):8-16
pubmed: 30866755
Mol Immunol. 2009 Oct;46(16):3245-50
pubmed: 19716177
J Infect Dis. 2010 Mar 15;201(6):923-35
pubmed: 20158392
Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17841-6
pubmed: 19805194
PLoS Pathog. 2008 Jul 04;4(7):e1000098
pubmed: 18604274
J Virol. 2012 Apr;86(7):3595-604
pubmed: 22258238
Br J Exp Pathol. 1948 Apr;29(2):107-21
pubmed: 18868961
PLoS Pathog. 2015 Sep 01;11(9):e1005143
pubmed: 26325027
Viruses. 2016 Jul 23;8(7):
pubmed: 27455310
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
PLoS One. 2017 Mar 9;12(3):e0173259
pubmed: 28278207
Int J Mol Sci. 2019 Jan 31;20(3):
pubmed: 30708982
Cell Mol Life Sci. 2017 Jun;74(11):2039-2054
pubmed: 28102430
J Microbiol Immunol Infect. 2017 Dec;50(6):747-754
pubmed: 28690024
J Cell Sci. 2015 Dec 1;128(23):4395-406
pubmed: 26490996
Front Cell Dev Biol. 2018 Feb 09;6:11
pubmed: 29479529
J Biol Chem. 2009 Apr 10;284(15):9835-44
pubmed: 19193639
Trends Biochem Sci. 2002 May;27(5):219-21
pubmed: 12076526
Nucleic Acids Res. 2016 Jan 4;44(D1):D279-85
pubmed: 26673716
Biochem Soc Trans. 2010 Feb;38(Pt 1):29-33
pubmed: 20074030
Cell Rep. 2017 Jan 17;18(3):762-776
pubmed: 28099853
Nucleic Acids Res. 2017 Jan 4;45(D1):D200-D203
pubmed: 27899674
Insect Biochem Mol Biol. 2012 Aug;42(8):545-56
pubmed: 22580186
Dev Comp Immunol. 2014 Mar;43(1):1-9
pubmed: 24135719
mSphere. 2017 May 3;2(3):
pubmed: 28497119
Int J Antimicrob Agents. 2003 Feb;21(2):153-7
pubmed: 12615379
PLoS Pathog. 2014 Feb 13;10(2):e1003931
pubmed: 24550728
Vet Res. 2010 Nov-Dec;41(6):61
pubmed: 21188836
J Med Entomol. 2018 Jan 10;55(1):78-89
pubmed: 28968775
PLoS One. 2010 Nov 03;5(11):e13805
pubmed: 21072193
mSphere. 2017 May 3;2(3):
pubmed: 28497117
PLoS Negl Trop Dis. 2015 Nov 13;9(11):e0004227
pubmed: 26566123
Nat Protoc. 2013 Aug;8(8):1494-512
pubmed: 23845962
J Am Mosq Control Assoc. 2014 Dec;30(4):253-9
pubmed: 25843130
Cell Microbiol. 2003 Jan;5(1):3-14
pubmed: 12542466
BMC Microbiol. 2008 Mar 17;8:47
pubmed: 18366655
J Gen Virol. 2016 May;97(5):1033-1036
pubmed: 26872460
Histochem Cell Biol. 2017 Feb;147(2):223-237
pubmed: 27999992
J Clin Microbiol. 2002 Jul;40(7):2323-30
pubmed: 12089242
PLoS Pathog. 2011 Jan 13;7(1):e1001252
pubmed: 21249175
Dev Comp Immunol. 2010 Apr;34(4):387-95
pubmed: 20026176
PLoS Pathog. 2006 Jun;2(6):e52
pubmed: 16789837
Cell. 2010 Sep 3;142(5):714-25
pubmed: 20797779
BMC Bioinformatics. 2019 Feb 18;20(1):84
pubmed: 30777018
J Med Entomol. 2008 Jan;45(1):102-8
pubmed: 18283949
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712