Immune blotting as a method for diagnosis of toxoplasmosis.
Иммунный блоттинг как метод диагностики токсоплазменной инфекции.
IgG-antibodies
Toxoplasma gondii
clinical laboratory diagnostics
enzyme-linked immunosorbent assay (ELISA)
immune blotting (IB)
toxoplasmosis
Journal
Klinicheskaia laboratornaia diagnostika
ISSN: 0869-2084
Titre abrégé: Klin Lab Diagn
Pays: Russia (Federation)
ID NLM: 9432021
Informations de publication
Date de publication:
04 Dec 2020
04 Dec 2020
Historique:
entrez:
10
12
2020
pubmed:
11
12
2020
medline:
28
1
2021
Statut:
ppublish
Résumé
Test kit for detection of IgG-antibodies to individual antigens of Toxoplasma gondii by immune blotting («Western blot» format) has been developed. Laboratory testing with first international WHO standard «Anti-toxoplasma serum (IgG), human, Lyophilized, 20 IU / ampoule» (NIBSC, Great Britain) demonstrated the analytical sensitivity of the new kit equal to 10 IU / ml. Study of the diagnostic efficiency of the new kit showed its high sensitivity, equal to 98.51 - 100%, and high specificity, equal to 99.5 - 100%. New test kit is intended for confirmatory testing in laboratory diagnostics of toxoplasmosis.
Identifiants
pubmed: 33301659
doi: 10.18821/0869-2084-2020-65-11-693-698
doi:
Substances chimiques
Antibodies, Protozoan
0
Immunoglobulin G
0
Reagent Kits, Diagnostic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
693-698Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Mardanly S.G., Simonova E.G., Simonov V.V. Torch-group infections: clinical laboratory diagnostics, epidemiological surveillance and control [Infektsiya Torch-gruppy: klinicheskaya laboratornaya diagnostika, epidemiologicheskiy nadzor i kontrol´]. Moscow: Tranzit-IKS; 2018. (in Russian)
Dolgikh T.I. Toxoplasmosis: modern strategies of laboratory diagnostics. Infektsiya i immunitet. 2011; 1(1): 43-50. (in Russian)
Kazantsev A.P. Toxoplasmosis.[Toksoplazmoz]. Leningrad: Meditsina; 1985. (in Russian)
Mardanly S.G. Epidemiological surveillance of TORCH-group infections based on modern technologies of laboratory diagnostics. Diss. Moscow; 2016. (in Russian)
Mardanly S.G., Kirpichnikova G.I., Neverov V.A. Toxoplasmosis: etiology, epidemiology, pathogenesis, clinical picture, laboratory diagnostics, prevention. [Toksoplazmoz: jetiologija, jepidemiologija, patogenez, klinika, laboratornaja diagnostika, profilaktika. Metodicheskoe posobie]. Jelektrogorsk: ZAO “JeKOlab”; 2005. (in Russian)
Gracheva L.I. Toxoplasmosis problem. Pediatriya. 1999; 4: 83-6. (in Russian)
Holec L., Hiszczynska-Sawicka E., Gasior A., Brillowska-Dabrowska A., Kur J. Use of MAG1 Recombinant Antigen for Diagnosis of Toxoplasma gondii Infection in Humans. Clin. Vaccine Immunol. 2007; 14(3): 220–5.
Robert-Gangneux F., Gavinet M.F., Ancelle T., Raymond J., Tourte-Schaefer C., Dupouy-Camet J. Value of prenatal diagnosis and early postnatal diagnosis of congenital toxoplasmosis: retrospective study of 110 cases. J. Clin. Microbiol. 1999; 37: 2893-8.
Mardanly S.G., Simonov V.V., Avdonina A.S. Production of reagent kits for clinical laboratory diagnostics by immunochemical methods. [Proizvodstvo naborov reagentov dlja klinicheskoj laboratornoj diagnostiki immunohimicheskimi metodami]. Orekhovo-Zuevo: GGTU; 2017. (in Russian)
Capobiango J.D., Monica T.C., Ferreira F.P., Mitsuka-Breganó R., Navarro I.T., Garcia J.L., Reiche E.M.V. Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis. J. Pediatr. (Rio J.). 2016; 92(6): 616-23.
Machado A.S., Andrade G.M.Q., Januário J.N., Fernandes M.D., Carneiro A.C.A.V, Carneiro M. et al. IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis. Mem. Inst. Oswaldo Cruz. 2010; 105(6): 757-61.
Magi B., Migliorini L. Western blotting for the diagnosis of congenital toxoplasmosis. New microbiologica. 2011; 34: 93-5.
Delibas S.B., Ertabaklar H., Ertug S. Evaluation of antigenic variations between two virulent toxoplasma strains. J. Med. Microbiol. 2006; 55: 1333–5.
Pfrepper K.-I., Enders G., Gohl M., Krczal D., Hlobil H., Wassenberg D., Soutschek E. Seroreactivity to and Avidity for Recombinant Antigens in Toxoplasmosis. Clin. Vaccine Immunol. 2005; 12(8): 977–82.
Chumpitazi B.F., Boussaid A., Pelloux H., Racinet C., Bost M, Goullier-Fleuret A. Diagnosis of congenital toxoplasmosis by immunoblotting and relationship with other methods. J. Clin. Microbiol. 1995; 33: 1479–85.
Gross U., Luder C.G., Hendgen V., Heeg C., Sauer I., Weidner A., Krczal D., Enders G. Comparative immunoglobulin G antibody profiles between mother and child (CGMC test) for early diagnosis of congenital toxoplasmosis. J. Clin. Microbiol. 2000; 38: 3619–22.
Nielsen H.V., Schmidt D.R., Petersen E. Diagnosis of Congenital Toxoplasmosis by Two-Dimensional Immunoblot Differentiation of Mother and Child Immunoglobulin G Profiles. J. Clin. Microbiol. 2005; 43(2): 711–5.
Rilling V., Dietz K., Krczal D., Knotek F., Enders G. Evaluation of a commercial IgG/IgM Western blot assay for early postnatal diagnosis of congenital toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 2003; 22: 174–80.
Robert-Gangneux F., Commerce V., Tourte-Schaefer C., DupouyCamet J. Performance of a Western blot assay to compare mother and newborn anti-Toxoplasma antibodies for the early neonatal diagnosis of congenital toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 1999; 18: 648–54.
Tissot Dupont D., Fricker-Hidalgo H., Brenier-Pinchart M.P., Bost-Bru C., Ambroise-Thomas P., Pelloux H. Usefulness of Western blot in serological follow-up of newborns suspected of congenital toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 2003; 22: 122–5.
Avdonina A.S., Mardanly S.G., Yuminova N.V. Development of an enzyme-linked immunosorbent assay system for detecting class M antibodies to individual proteins of human cytomegalovirus by immune blotting in the “Western blot” format. Vestnik sovremennoy klinicheskoy meditsiny. 2016; 9 (5): 7-14. (in Russian)
Mardanly S.G., Avdonina A.S., Mardanly S.S., Shershneva N.N. Line-Blot. Meditsinskiy alfavit. 2017; 4 (38): 33-40. (in Russian)
Mardanly S.G., Avdonina A.S. Development of a set of reagents for detecting class G antibodies to individual rubella antigens by immune blotting in the “Western blot” format. Klinicheskaya laboratornaya diagnostika. 2018; 63 (11): 696-701. (in Russian)
Mardanly S.G., Avdonina A.S. Development of a set of reagents for the detection of class G antibodies to individual cytomegalovirus antigens by immune blotting in the Western Blot format. Klinicheskaya dermatologiya i venerologiya. 2014; 1: 18-24. (in Russian)
Arsenyeva V.A., Amelina E.A., Mardanly S.G., Rotanov S.V. Linear immunoblotting for the simultaneous detection of antibodies to the main pathogens of the TORCH group. Meditsinskiy alfavit. 2017; 20(317): 43-8. (in Russian)
Antonov V.S. Questions of statistical evaluation of the results of clinical trials of medical devices for in vitro diagnostics. Available at: https://docplayer.ru/118768-Voprosy-statisticheskoy-otsenki-rezul´tatov-klinicheskikh-ispytaniy-meditsinskikh-izdeliy-dlya-in-vitro-diagnostiki-antonov-vs-zamestitel-generalnogo (Accessed 28 September 2020). (in Russian)
Chernov V.I., Esaulenko I.E., Rodionov O.V., Semenov S.N. Medical informatics: a tutorial.[ Meditsinskaya informatika: uchebnoe posobie]. Rostov- na- Donu: Feniks; 2007. (in Russian)
Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227: 680-5.