ZBTB2 protein is a new partner of the Nucleosome Remodeling and Deacetylase (NuRD) complex.
Adenosine Triphosphatases
/ metabolism
Cell Line, Tumor
Chromatin
/ genetics
Chromosomal Proteins, Non-Histone
/ metabolism
DNA
/ genetics
DNA Methylation
DNA-Binding Proteins
/ metabolism
Glioblastoma
/ metabolism
Humans
Immunoprecipitation
/ methods
Mass Spectrometry
/ methods
Mi-2 Nucleosome Remodeling and Deacetylase Complex
/ genetics
Nuclear Proteins
/ genetics
Nucleosomes
/ genetics
Protein Binding
/ genetics
Repressor Proteins
/ genetics
Transcription Factors
/ metabolism
Zinc Fingers
/ physiology
BTB-POZ zinc-finger
Chromatin remodeling complexes
High-resolution mass spectrometry
NuRD
ZBTB2
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
31 Jan 2021
31 Jan 2021
Historique:
received:
06
10
2020
revised:
25
11
2020
accepted:
04
12
2020
pubmed:
11
12
2020
medline:
28
4
2021
entrez:
10
12
2020
Statut:
ppublish
Résumé
ZBTB2 is a protein belonging to the BTB/POZ zinc-finger family whose members typically contain a BTB/POZ domain at the N-terminus and several zinc-finger domains at the C-terminus. Studies have been carried out to disclose the role of ZBTB2 in cell proliferation, in human cancers and in regulating DNA methylation. Moreover, ZBTB2 has been also described as an ARF, p53 and p21 gene repressor as well as an activator of genes modulating pluripotency. In this scenario, ZBTB2 seems to play many functions likely associated with other proteins. Here we report a picture of the ZBTB2 protein partners in U87MG cell line, identified by high-resolution mass spectrometry (MS) that highlights the interplay between ZBTB2 and chromatin remodeling multiprotein complexes. In particular, our analysis reveals the presence, as ZBTB2 candidate interactors, of SMARCA5 and BAZ1B components of the chromatin remodeling complex WICH and PBRM1, a subunit of the SWI/SNF complex. Intriguingly, we identified all the subunits of the NuRD complex among the ZBTB2 interactors. By co-immunoprecipitation experiments and ChIP-seq analysis we definitely identify ZBTB2 as a new partner of the NuRD complex.
Identifiants
pubmed: 33301849
pii: S0141-8130(20)35187-4
doi: 10.1016/j.ijbiomac.2020.12.029
pii:
doi:
Substances chimiques
BAZ1B protein, human
0
Chromatin
0
Chromosomal Proteins, Non-Histone
0
DNA-Binding Proteins
0
Nuclear Proteins
0
Nucleosomes
0
PBRM1 protein, human
0
Repressor Proteins
0
Transcription Factors
0
ZBTB2 protein, human
0
DNA
9007-49-2
Mi-2 Nucleosome Remodeling and Deacetylase Complex
EC 3.5.1.98
Adenosine Triphosphatases
EC 3.6.1.-
SMARCA5 protein, human
EC 3.6.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
67-76Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest None.