Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition.
Electron microscopy
Exosomes
LC–MS/MS
Proteins
Secretion
Size exclusion chromatography
Journal
International journal for parasitology
ISSN: 1879-0135
Titre abrégé: Int J Parasitol
Pays: England
ID NLM: 0314024
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
18
08
2020
revised:
23
09
2020
accepted:
28
09
2020
pubmed:
12
12
2020
medline:
12
10
2021
entrez:
11
12
2020
Statut:
ppublish
Résumé
We provided the first known evidence of the presence and release of extracellular vesicles in adults of important model tapeworm Hymenolepis diminuta. Two different subtypes have been observed on the surface of the worm and among the secretory products confirmed by several microscopical methods. Proteomic analysis revealed the presence of parasite-specific proteins as well as those of the host in purified extracellular vesicles. Among the protein cargo, we identified potential drug targets, vaccine candidates and H. diminuta antigens. Finally, the protein composition further revealed proteins participating in the endosomal complex required for transport-dependent biogenesis pathway.
Identifiants
pubmed: 33307002
pii: S0020-7519(20)30317-9
doi: 10.1016/j.ijpara.2020.09.010
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
327-332Informations de copyright
Copyright © 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.