Detection of SARS-CoV-2 N-antigen in blood during acute COVID-19 provides a sensitive new marker and new testing alternatives.
Antigen
Antigenemia
Blood
COVID-19
Diagnostic
Plasma
SARS-CoV-2
Serum
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
ISSN: 1469-0691
Titre abrégé: Clin Microbiol Infect
Pays: England
ID NLM: 9516420
Informations de publication
Date de publication:
08 12 2020
08 12 2020
Historique:
received:
05
10
2020
revised:
23
11
2020
accepted:
24
11
2020
entrez:
11
12
2020
pubmed:
12
12
2020
medline:
12
12
2020
Statut:
aheadofprint
Résumé
Molecular assays on nasopharyngeal swabs remain the cornerstone of COVID-19 diagnostic. The high technicalities of nasopharyngeal sampling and molecular assays, as well as scarce resources of reagents, limit our testing capabilities. Several strategies failed, to date, to fully alleviate this testing process (e.g. saliva sampling or antigen testing on nasopharyngeal samples). We assessed the clinical performances of SARS-CoV-2 nucleocapsid antigen (N-antigen) ELISA detection in serum or plasma using the COVID-19 Quantigene® (AAZ, France) assay. Performances were determined on 63 sera from 63 non-COVID patients and 227 serum samples (165 patients) from the French COVID and CoV-CONTACT cohorts with RT-PCR confirmed SARS-CoV-2 infection, including 142 serum (114 patients) obtained within 14 days after symptoms' onset. Specificity was 98.4% (95% confidence interval [CI], 95.3 to 100). Sensitivity was 79.3% overall (180/227, 95% CI, 74.0 to 84.6) and 93.0% (132/142, 95% CI, 88.7 to 97.2) within 14 days after symptoms onset. 91 included patients had a sera and nasopharyngeal swabs collected in the same 24 hours. Among those with high nasopharyngeal viral loads, i.e. Ct value below 30 and 33, only 1/50 and 4/67 tested negative for N-antigenemia, respectively. Among those with a negative nasopharyngeal RT-PCR, 8/12 presented positive N-antigenemia; the lower respiratory tract was explored for 6 of these 8 patients, showing positive RT-PCR in 5 cases. This is the first evaluation of a commercially available serum N-antigen detection assay. It presents a robust specificity and sensitivity within the first 14 days after symptoms onset. This approach provides a valuable new option for COVID-19 diagnosis, only requiring a blood draw and easily scalable in all clinical laboratories.
Identifiants
pubmed: 33307227
pii: S1198-743X(20)30721-7
doi: 10.1016/j.cmi.2020.11.025
pmc: PMC7724284
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Investigateurs
Christophe Choquet
(C)
Jean-François Timsit
(JF)
Jade Ghosn
(J)
Charlotte Charpentier
(C)
Diane Descamps
(D)
Nadhira Houhou-Fidouh
(N)
Alpha Diallo
(A)
Soizic Le Mestre
(S)
Noémie Mercier
(N)
Christelle Paul
(C)
Ventzislava Petrov-Sanchez
(V)
Denis Malvy
(D)
Catherine Chirouze
(C)
Claire Andrejak
(C)
François Dubos
(F)
Patrick Rossignol
(P)
Olivier Picone
(O)
François Bompart
(F)
Tristan Gigante
(T)
Morgane Gilg
(M)
Bénédicte Rossignol
(B)
Claire Levy-Marchal
(C)
Marine Beluze
(M)
Jean Sébastien Hulot
(JS)
Delphine Bachelet
(D)
Krishna Bhavsar
(K)
Lila Bouadma
(L)
Anissa Chair
(A)
Camille Couffignal
(C)
Charlene Da Silveira
(C)
Marie Pierre Debray
(MP)
Diane Descamps
(D)
Xavier Duval
(X)
Philippine Eloy
(P)
Marina Esposito-Farese
(M)
Nadia Ettalhaoui
(N)
Nathalie Gault
(N)
Jade Ghosn
(J)
Isabelle Gorenne
(I)
Isabelle Hoffmann
(I)
Ouifiya Kafif
(O)
Sabrina Kali
(S)
Antoine Khalil
(A)
Cédric Laouénan
(C)
Samira Laribi
(S)
Minh Le
(M)
Quentin Le Hingrat
(Q)
François-Xavier Lescure
(FX)
Jean Christophe Lucet
(JC)
France Mentré
(F)
Jimmy Mullaert
(J)
Nathan Peiffer-Smadja
(N)
Gilles Peytavin
(G)
Carine Roy
(C)
Marion Schneider
(M)
Nassima Si Mohammed
(NS)
Lysa Tagherset
(L)
Coralie Tardivon
(C)
Marie Capucine Tellier
(MC)
Jean-François Timsit
(JF)
Théo Trioux
(T)
Sarah Tubiana
(S)
Benoit Visseaux
(B)
Noémie Vanel
(N)
Romain Basmaci
(R)
François Angoulvant
(F)
Florentia Kaguelidou
(F)
Justine Pages
(J)
Christelle Tual
(C)
Aurélie Veislinger
(A)
Sandrine Couffin-Cardiergues
(S)
Hélène Esperou
(H)
Ikram Houas
(I)
Salma Jaafoura
(S)
Aurélie Papadopoulos
(A)
Alexandra Coelho
(A)
Alphonsine Diouf
(A)
Alexandre Hoctin
(A)
Marina Mambert
(M)
Maude Bouscambert
(M)
Alexandre Gaymard
(A)
Bruno Lina
(B)
Manuel Rosa-Calatrava
(M)
Olivier Terrier
(O)
Dehbia Benkerrou
(D)
Céline Dorival
(C)
Amina Meziane
(A)
François Téoulé
(F)
Jérémie Guedj
(J)
Hervé Le Nagard
(H)
Guillaume Lingas
(G)
Nadège Neant
(N)
Laurent Abel
(L)
Mathilde Desvallée
(M)
Coralie Khan
(C)
Dominique Deplanque
(D)
Yazdan Yazdanpanah
(Y)
Sylvie Behilill
(S)
Vincent Enouf
(V)
Hugo Mouquet
(H)
Sylvie Van Der Werf
(S)
Minerva Cervantes-Gonzalez
(M)
Eric D'ortenzio
(E)
Oriane Puéchal
(O)
Caroline Semaille
(C)
Marion Noret
(M)
Manuel Etienne
(M)
Yves Levy
(Y)
Aurélie Wiedemann
(A)
Duval Xavier
(D)
Burdet Charles
(B)
Duval Xavier
(D)
Lina Bruno
(L)
Tubiana Sarah
(T)
Van Der Werf Sylvie
(V)
Abad Fanny
(A)
Abry Dominique
(A)
Alavoine Loubna
(A)
Allain Jean-Sébastien
(A)
Amiel-Taieb Karline
(AT)
Audoin Pierre
(A)
Augustin Shana
(A)
Ayala Sandrine
(A)
Bansard Hélène
(B)
Bertholon Fréderique
(B)
Boissel Nolwenn
(B)
Botelho-Nevers Elisabeth
(BN)
Bouiller Kévin
(B)
Bourgeon Marilou
(B)
Boutrou Mathilde
(B)
Brick Lysiane
(B)
Bruneau Léa
(B)
Caumes Eric
(C)
Chabouis Agnès
(C)
Chan Thien Eric
(CT)
Chirouze Catherine
(C)
Coignard Bruno
(C)
Costa Yolande
(C)
Costenoble Virginie
(C)
Cour Sylvie
(C)
Cracowski Claire
(C)
Cracowski Jean Luc
(CJ)
Deplanque Dominique
(D)
Dequand Stéphane
(D)
Desille-Dugast Mireille
(DD)
Desmarets Maxime
(D)
Detoc Maelle
(D)
Dewitte Marie
(D)
Djossou Felix
(D)
Ecobichon Jean-Luc
(E)
Elrezzi Elise
(E)
Faurous William
(F)
Fortuna Viviane
(F)
Fouchard Julie
(F)
Gantier Emilie
(G)
Gautier Céline
(G)
Gerardin Patrick
(G)
Gerset Sandrine
(G)
Gilbert Marie
(G)
Gissot Valérie
(G)
Guillemin Francis
(G)
Hartard Cédric
(H)
Hazevis Béatrice
(H)
Hocquet Didier
(H)
Hodaj Enkelejda
(H)
Ilic-Habensus Emila
(IH)
Jeudy A
(J)
Jeulin Helene
(J)
Kane Maty
(K)
Kasprzyk Emmanuelle
(K)
Kikoine John
(K)
Laine Fabrice
(L)
Laviolle Bruno
(L)
Lebeaux David
(L)
Leclercq Anne
(L)
Ledru Eric
(L)
Lefevre Benjamin
(L)
Legoas Carole
(L)
Legrand Amélie
(L)
Legrand Karine
(L)
Lehacaut Jonathan
(L)
Lehur Claire
(L)
Lemouche Dalila
(L)
Lepiller Quentin
(L)
Lepuil Sévérine
(L)
Letienne Estelle
(L)
Lucarelli Aude
(L)
Lucet Jean-Christophe
(L)
Madeline Isabelle
(M)
Maillot Adrien
(M)
Malapate Catherine
(M)
Malvy Denis
(M)
Mandic Milica
(M)
Marty-Quinternet Solène
(MQ)
Meghadecha Mohamed
(M)
Mergeay-Fabre Mayka
(MF)
Mespoulhe Pauline
(M)
Meunier Alexandre
(M)
Migaud Maria-Claire
(M)
Motiejunaite Justina
(M)
Gay Nathalie
(G)
Nguyen Duc
(N)
Oubbea Soumaya
(O)
Pagadoy Maïder
(P)
Paris Adeline
(P)
Paris Christophe
(P)
Payet Christine
(P)
Peiffer-Smadja Nathan
(PS)
Perez Lucas
(P)
Perreau Pauline
(P)
Pierrez Nathalie
(P)
Pistone Thierry
(P)
Postolache Andreea
(P)
Rasoamanana Patrick
(R)
Reminiac Cécile
(R)
Rexah Jade
(R)
Roche-Gouanvic Elise
(RG)
Rousseau Alexandra
(R)
Schoemaecker Betty
(S)
Simon Sandrine
(S)
Soler Catherine
(S)
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(S)
Sow Khaly
(S)
Tardy Bernard
(T)
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(T)
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(T)
Tournier Anne
(T)
Tyrode Sandrine
(T)
Vauchy Charline
(V)
Verdon Renaud
(V)
Vernet Pauline
(V)
Vignali Valérie
(V)
Waucquier Nawal
(W)
Burdet Charles
(B)
Do Thi Thu Huong
(DT)
Laouénan Cédric
(L)
Mentre France
(M)
Pauline Manchon
(P)
Tubiana Sarah
(T)
Dechanet Aline
(D)
Letrou Sophie
(L)
Quintin Caroline
(Q)
Frezouls Wahiba
(F)
Le Hingrat Quentin
(LH)
Houhou Nadhira
(H)
Damond Florence
(D)
Descamps Dianes
(D)
Charpentier Charlotte
(C)
Visseaux Benoit
(V)
Vabret Astrid
(V)
Lina Bruno
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Van Der Werf Sylvie
(V)
Behillil Sylvie
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Gaillanne Laurence
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Benmalek Nabil
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Attia Mikael
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Barbet Marion
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Demeret Caroline
(D)
Rose Thierry
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Petres Stéphane
(P)
Escriou Nicolas
(E)
Barbet Marion
(B)
Petres Stéphane
(P)
Escriou Nicolas
(E)
Goyard Sophie
(G)
Kafif Ouifiya
(K)
Piquard Valentine
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Tubiana Sarah
(T)
Coignard Bruno
(C)
Mailles Alexandra
(M)
Simondon Anne
(S)
Dreyere Marion
(D)
Morel Bruno
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Vesval Thiphaine
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None Inserm
Amat Karine
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Ammour Douae
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Aqourras Khadija
(A)
Couffin-Cadiergues Sandrine
(CC)
Delmas Christelle
(D)
Desan Vristi
(D)
Doute Jean Michel
(DJ)
Esperou Hélène
(E)
Hendou Samia
(H)
Kouakam Christelle
(K)
Le Meut Guillaume
(LM)
Lemestre Soizic
(L)
Leturque Nicolas
(L)
Marcoul Emmanuelle
(M)
Nguefang Solange
(N)
Roufai Layidé
(R)
Laurent Abel
(L)
Caillat-Zucman Sophie
(CZ)
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.
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