Mapping the Endothelial Cell

Animals Chromatography, High Pressure Liquid Cystathionine gamma-Lyase / genetics Cysteine / chemistry Disulfides / analysis Endothelial Cells / cytology Humans Hydrogen Sulfide / pharmacology Integrin beta Chains / chemistry Mechanotransduction, Cellular Mice Shear Strength Sulfhydryl Compounds / chemistry Tandem Mass Spectrometry Vasodilation / drug effects rhoA GTP-Binding Protein / metabolism
cystathionine cysteine endothelial cells hydrogen sulfide integrins mechanotransduction, cellular

Journal

Circulation
ISSN: 1524-4539
Titre abrégé: Circulation
Pays: United States
ID NLM: 0147763

Informations de publication

Date de publication:
02 03 2021
Historique:
pubmed: 15 12 2020
medline: 4 1 2022
entrez: 14 12 2020
Statut: ppublish

Résumé

In vascular endothelial cells, cysteine metabolism by the cystathionine γ lyase (CSE), generates hydrogen sulfide-related sulfane sulfur compounds (H Liquid chromatography with tandem mass spectrometry was used to identify Three paired sample sets were compared: (1) native human endothelial cells isolated from plaque-free mesenteric arteries (CSE activity high) and plaque-containing carotid arteries (CSE activity low); (2) cultured human endothelial cells kept under static conditions or exposed to fluid shear stress to decrease CSE expression; and (3) cultured endothelial cells exposed to shear stress to decrease CSE expression and treated with solvent or the slow-releasing H Vascular disease is associated with marked changes in the

Sections du résumé

BACKGROUND
In vascular endothelial cells, cysteine metabolism by the cystathionine γ lyase (CSE), generates hydrogen sulfide-related sulfane sulfur compounds (H
METHODS
Liquid chromatography with tandem mass spectrometry was used to identify
RESULTS
Three paired sample sets were compared: (1) native human endothelial cells isolated from plaque-free mesenteric arteries (CSE activity high) and plaque-containing carotid arteries (CSE activity low); (2) cultured human endothelial cells kept under static conditions or exposed to fluid shear stress to decrease CSE expression; and (3) cultured endothelial cells exposed to shear stress to decrease CSE expression and treated with solvent or the slow-releasing H
CONCLUSIONS
Vascular disease is associated with marked changes in the

Identifiants

DOI: 10.1161/CIRCULATIONAHA.120.051877 PMID: 33307764
pubmed: 33307764
doi: 10.1161/CIRCULATIONAHA.120.051877
doi:

Substances chimiques

Disulfides 0
Integrin beta Chains 0
Sulfhydryl Compounds 0
rhoA GTP-Binding Protein EC 3.6.5.2
Cystathionine gamma-Lyase EC 4.4.1.1
Cysteine K848JZ4886
Hydrogen Sulfide YY9FVM7NSN

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

935-948

Auteurs

Sofia-Iris Bibli (SI)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Jiong Hu (J)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Mario Looso (M)

German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).
Bioinformatics Core Unit (M.L., P.G.), Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Andreas Weigert (A)

Institute of Biochemistry I, Faculty of Medicine, Goethe-University Frankfurt, Germany (A.W.).

Corina Ratiu (C)

Centre for Molecular Medicine, Institute for Cardiovascular Physiology (C.R., M.S.L., R.P.B.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Janina Wittig (J)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Maria Kyriaki Drekolia (MK)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.

Lukas Tombor (L)

Institute for Cardiovascular Regeneration (L.T., S.D.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Voahanginirina Randriamboavonjy (V)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Matthias S Leisegang (MS)

Centre for Molecular Medicine, Institute for Cardiovascular Physiology (C.R., M.S.L., R.P.B.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Philipp Goymann (P)

German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).
Bioinformatics Core Unit (M.L., P.G.), Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Fredy Delgado Lagos (F)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Beate Fisslthaler (B)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Sven Zukunft (S)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Anastasia Kyselova (A)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Alberto Fernando Oliveira Justo (AFO)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Juliana Heidler (J)

Functional Proteomics (J.Heidler., I.W.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Diamantis Tsilimigras (D)

First Propedeutic Department of Surgery, Vascular Surgery Division (D.T., F.S.), National and Kapodistrian University of Athens Medical School, Greece.

Ralf P Brandes (RP)

Centre for Molecular Medicine, Institute for Cardiovascular Physiology (C.R., M.S.L., R.P.B.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Stefanie Dimmeler (S)

Institute for Cardiovascular Regeneration (L.T., S.D.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

Andreas Papapetropoulos (A)

Laboratory of Pharmacology, Faculty of Pharmacy (A.P.), National and Kapodistrian University of Athens Medical School, Greece.
Clinical, Experimental Surgery and Translational Research Center, Biomedical Research Foundation of the Academy of Athens, Greece (A.P.).

Stefan Knapp (S)

Institute for Pharmaceutical Chemistry and Buchmann Institute for Molecular Life Sciences (S.K.), Goethe University, Frankfurt am Main, Germany.

Stefan Offermanns (S)

German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).
Department of Pharmacology (S.O.), Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Ilka Wittig (I)

Functional Proteomics (J.Heidler., I.W.), Goethe University, Frankfurt am Main, Germany.

Stephen L Nishimura (SL)

Department of Pathology, University of California San Francisco (S.L.N.).

Fragiska Sigala (F)

First Propedeutic Department of Surgery, Vascular Surgery Division (D.T., F.S.), National and Kapodistrian University of Athens Medical School, Greece.

Ingrid Fleming (I)

Institute for Vascular Signalling (S-I.B., J.H., J.W., M.K.D., V.R., F.D.L., B.F., S.Z., A.K., A.F.O.J., I.F.), Goethe University, Frankfurt am Main, Germany.
German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany (S-I.B., J.Hu, M.L., C.R., J.W., L.T., V.R., M.S.L., P.G., F.D.L., B.F., S.Z., A.K., A.F.O.J., J.Heidler, R.P.B., S.D., S.O., I.W., I.F.).

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Mapping the Endothelial Cell
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Mapping the Endothelial Cell
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-sulfur lyases › Cystathionine gamma-lyase › Mapping the Endothelial Cell
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés soufrés › Cystéine › Mapping the Endothelial Cell
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Sulfures › Disulfures › Mapping the Endothelial Cell
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › Mapping the Endothelial Cell
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Mapping the Endothelial Cell
questionsmedicales.fr › Produits chimiques inorganiques › Sulfure d'hydrogène › Mapping the Endothelial Cell
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Intégrines › Chaines bêta des intégrines › Mapping the Endothelial Cell
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Mécanotransduction cellulaire › Mapping the Endothelial Cell
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Mapping the Endothelial Cell
questionsmedicales.fr › Phénomènes physiques › Phénomènes mécaniques › Résistance au cisaillement › Mapping the Endothelial Cell
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Thiols › Mapping the Endothelial Cell
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Mapping the Endothelial Cell
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiologiques cardiovasculaires › Hémodynamique › Vasodilatation › Mapping the Endothelial Cell
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › Protéines G monomériques › Protéines G rho › Protéine G RhoA › Mapping the Endothelial Cell