Deep learning for sensitive detection of Helicobacter Pylori in gastric biopsies.

Artificial intelligence Convolutional neural networks Deep learning Gastric cancer prevention Helicobacter pylori Screening

Journal

BMC gastroenterology
ISSN: 1471-230X
Titre abrégé: BMC Gastroenterol
Pays: England
ID NLM: 100968547

Informations de publication

Date de publication:
11 Dec 2020
Historique:
received: 02 12 2019
accepted: 08 10 2020
entrez: 14 12 2020
pubmed: 15 12 2020
medline: 15 5 2021
Statut: epublish

Résumé

Helicobacter pylori, a 2 × 1 μm spiral-shaped bacterium, is the most common risk factor for gastric cancer worldwide. Clinically, patients presenting with symptoms of gastritis, routinely undergo gastric biopsies. The following histo-morphological evaluation dictates therapeutic decisions, where antibiotics are used for H. pylori eradication. There is a strong rational to accelerate the detection process of H. pylori on histological specimens, using novel technologies, such as deep learning. We designed a deep-learning-based decision support algorithm that can be applied on regular whole slide images of gastric biopsies. In detail, we can detect H. pylori both on Giemsa- and regular H&E stained whole slide images. With the help of our decision support algorithm, we show an increased sensitivity in a subset of 87 cases that underwent additional PCR- and immunohistochemical testing to define a sensitive ground truth of HP presence. For Giemsa stained sections, the decision support algorithm achieved a sensitivity of 100% compared to 68.4% (microscopic diagnosis), with a tolerable specificity of 66.2% for the decision support algorithm compared to 92.6 (microscopic diagnosis). Together, we provide the first evidence of a decision support algorithm proving as a sensitive screening option for H. pylori that can potentially aid pathologists to accurately diagnose H. pylori presence on gastric biopsies.

Sections du résumé

BACKGROUND BACKGROUND
Helicobacter pylori, a 2 × 1 μm spiral-shaped bacterium, is the most common risk factor for gastric cancer worldwide. Clinically, patients presenting with symptoms of gastritis, routinely undergo gastric biopsies. The following histo-morphological evaluation dictates therapeutic decisions, where antibiotics are used for H. pylori eradication. There is a strong rational to accelerate the detection process of H. pylori on histological specimens, using novel technologies, such as deep learning.
METHODS METHODS
We designed a deep-learning-based decision support algorithm that can be applied on regular whole slide images of gastric biopsies. In detail, we can detect H. pylori both on Giemsa- and regular H&E stained whole slide images.
RESULTS RESULTS
With the help of our decision support algorithm, we show an increased sensitivity in a subset of 87 cases that underwent additional PCR- and immunohistochemical testing to define a sensitive ground truth of HP presence. For Giemsa stained sections, the decision support algorithm achieved a sensitivity of 100% compared to 68.4% (microscopic diagnosis), with a tolerable specificity of 66.2% for the decision support algorithm compared to 92.6 (microscopic diagnosis).
CONCLUSION CONCLUSIONS
Together, we provide the first evidence of a decision support algorithm proving as a sensitive screening option for H. pylori that can potentially aid pathologists to accurately diagnose H. pylori presence on gastric biopsies.

Identifiants

pubmed: 33308189
doi: 10.1186/s12876-020-01494-7
pii: 10.1186/s12876-020-01494-7
pmc: PMC7731757
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

417

Subventions

Organisme : Else Kröner-Fresenius-Stiftung
ID : 2015_Kolleg.19

Références

Cochrane Database Syst Rev. 2015 Jul 22;(7):CD005583
pubmed: 26198377
Endosc Int Open. 2018 Feb;6(2):E139-E144
pubmed: 29399610
CA Cancer J Clin. 2019 Jan;69(1):7-34
pubmed: 30620402
BMJ. 1991 Jun 1;302(6788):1302-5
pubmed: 2059685
J Infect Dis. 1993 Aug;168(2):379-85
pubmed: 8335974
JAMA. 2013 Apr 3;309(13):1351-2
pubmed: 23549579
N Engl J Med. 1995 Jul 6;333(1):32-41
pubmed: 7776992
Nat Rev Gastroenterol Hepatol. 2017 Apr;14(4):230-240
pubmed: 28053340
Am J Gastroenterol. 2007 Aug;102(8):1808-25
pubmed: 17608775
EBioMedicine. 2017 Nov;25:106-111
pubmed: 29056541
N Engl J Med. 1991 Oct 17;325(16):1132-6
pubmed: 1891021
Gut. 2012 May;61(5):646-64
pubmed: 22491499
Lancet. 2019 May 11;393(10184):1948-1957
pubmed: 30982686
Arch Pathol Lab Med. 2020 Mar;144(3):370-378
pubmed: 31246112
Endoscopy. 2004 Jul;36(7):601-8
pubmed: 15243882
Am J Gastroenterol. 2005 May;100(5):1037-42
pubmed: 15842576
Cancer Cell. 2018 Jan 8;33(1):137-150.e5
pubmed: 29290541
Nature. 2015 May 28;521(7553):436-44
pubmed: 26017442
Gastroenterology. 2009 Nov;137(5):1641-8.e1-2
pubmed: 19664631
IARC Monogr Eval Carcinog Risks Hum. 1994;61:1-241
pubmed: 7715068
N Engl J Med. 1991 Oct 17;325(16):1127-31
pubmed: 1891020

Auteurs

Sebastian Klein (S)

Else-Kröner-Forschungskolleg, Clonal Evolution in Cancer, University Hospital Cologne, Cologne, Germany. Sebastian.Klein@uk-koeln.de.
Institute for Pathology, University Hospital Cologne, Cologne, Germany. Sebastian.Klein@uk-koeln.de.

Jacob Gildenblat (J)

DeePathology.ai, Raanana, Israel.

Michaele Angelika Ihle (MA)

Institute for Pathology, University Hospital Cologne, Cologne, Germany.

Sabine Merkelbach-Bruse (S)

Institute for Pathology, University Hospital Cologne, Cologne, Germany.

Ka-Won Noh (KW)

Institute for Pathology, University Hospital Cologne, Cologne, Germany.

Martin Peifer (M)

Department of Translational Genomics, Center of Integrated Oncology Cologne-Bonn, Medical Faculty, University of Cologne, 50931, Cologne, Germany.

Alexander Quaas (A)

Institute for Pathology, University Hospital Cologne, Cologne, Germany.

Reinhard Büttner (R)

Institute for Pathology, University Hospital Cologne, Cologne, Germany. Reinhard.Buettner@uk-koeln.de.

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