Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions.
Artificial basement membranes
endothelial cell function
thickness
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078
Informations de publication
Date de publication:
10 Mar 2021
10 Mar 2021
Historique:
pubmed:
15
12
2020
medline:
5
10
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues.
Identifiants
pubmed: 33310992
doi: 10.2116/analsci.20SCP10
pii: 10.2116/analsci.20SCP10
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
491-495Références
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