Direct RNA sequencing dataset of SMG1 KO mutant Physcomitrella (
Direct RNA sequencing
Nonsense-mediated decay
Physcomitrella (Physcomitrium patens)
SMG1 knockout
Transcriptomics
Journal
Data in brief
ISSN: 2352-3409
Titre abrégé: Data Brief
Pays: Netherlands
ID NLM: 101654995
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
03
11
2020
revised:
23
11
2020
accepted:
24
11
2020
entrez:
14
12
2020
pubmed:
15
12
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Nonsense-mediated mRNA decay (NMD) is a system that controls the quality of mRNA transcripts in eukaryotes by degradation of aberrant transcripts in a pioneer round of translation. In mammals, NMD targets one-third of mutated, disease-causing mRNAs and ∼10% of unmutated mRNAs, facilitating appropriate cellular responses to environmental changes [1]. In plants, NMD plays an important role in development and regulating abiotic and biotic stress responses [2]. The transcripts with premature termination codons (PTCs), upstream ORFs or long 3'-UTRs can be targeted to NMD. It was shown that alternative splicing plays a crucial role in regulation of NMD triggering, for example, by the introduction of a PTC in transcripts. Therefore, the correct identification of mRNA isoforms is a key step in the study of the principles of regulation of the cell transcriptome by the NMD pathway. Here, we performed long-read sequencing of Physcomitrella (
Identifiants
pubmed: 33313367
doi: 10.1016/j.dib.2020.106602
pii: S2352-3409(20)31482-7
pmc: PMC7721605
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106602Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article.
Références
Bioinformatics. 2019 Feb 1;35(3):523-525
pubmed: 30052755
Plant Cell Physiol. 2019 Sep 1;60(9):1953-1960
pubmed: 31111919
Elife. 2020 Jan 14;9:
pubmed: 31931956
Nucleic Acids Res. 2018 Jun 20;46(11):5822-5836
pubmed: 29596649
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Plant J. 2013 Dec;76(5):800-10
pubmed: 24103012
Nat Rev Mol Cell Biol. 2019 Jul;20(7):406-420
pubmed: 30992545
Cold Spring Harb Protoc. 2009 Feb;2009(2):pdb.prot5136
pubmed: 20147066
Bioinformatics. 2018 Sep 15;34(18):3094-3100
pubmed: 29750242