Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.
DRAQ5
EVCM
Mohs
acridine orange
confocal microscopy
tissue fixture
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
revised:
10
12
2020
received:
29
05
2020
accepted:
11
12
2020
pubmed:
15
12
2020
medline:
13
7
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
We investigated the utility of the fluorescent dye Deep Red Anthraquinone 5 (DRAQ5) for digital staining of optically sectioned skin in comparison to acridine orange (AO). Eight fresh-frozen thawed Mohs discard tissue specimens were stained with AO and DRAQ5, and imaged using an ex vivo confocal microscope at three wavelengths (488 nm and 638 nm for fluorescence, 785 nm for reflectance). Images were overlaid (AO + Reflectance, DRAQ5 + Reflectance), digitally stained, and evaluated by three investigators for perceived image quality (PIQ) and histopathological feature identification. In addition to nuclear staining, AO seemed to stain dermal fibers in a subset of cases in digitally stained images, while DRAQ5 staining was more specific to nuclei. Blinded evaluation showed substantial agreement, favoring DRAQ5 for PIQ (82%, Cl 75%-90%, Gwet's AC 0.74) and for visualization of histopathological features in (81%, Cl 73%-89%, Gwet's AC 0.67), supporting its use in digital staining of multimodal confocal micrographs of skin.
Identifiants
pubmed: 33314673
doi: 10.1002/jbio.202000207
pmc: PMC8274380
mid: NIHMS1717419
doi:
Substances chimiques
Anthraquinones
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000207Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB020029
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01EB020029
Pays : United States
Informations de copyright
© 2020 Wiley-VCH GmbH.
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