Diminishing effects of mechanical loading over time during rat Achilles tendon healing.
Achilles Tendon
/ drug effects
Animals
Biomechanical Phenomena
/ drug effects
Botulinum Toxins, Type A
/ physiology
Collagen
/ pharmacology
Disease Models, Animal
Female
Rats
Rats, Sprague-Dawley
Scattering, Small Angle
Stress, Mechanical
Tendon Injuries
/ drug therapy
Wound Healing
/ drug effects
X-Ray Diffraction
/ methods
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
06
2020
accepted:
23
11
2020
entrez:
14
12
2020
pubmed:
15
12
2020
medline:
12
1
2021
Statut:
epublish
Résumé
Mechanical loading affects tendon healing and recovery. However, our understanding about how physical loading affects recovery of viscoelastic functions, collagen production and tissue organisation is limited. The objective of this study was to investigate how different magnitudes of loading affects biomechanical and collagen properties of healing Achilles tendons over time. Achilles tendon from female Sprague Dawley rats were cut transversely and divided into two groups; normal loading (control) and reduced loading by Botox (unloading). The rats were sacrificed at 1, 2- and 4-weeks post-injury and mechanical testing (creep test and load to failure), small angle x-ray scattering (SAXS) and histological analysis were performed. The effect of unloading was primarily seen at the early time points, with inferior mechanical and collagen properties (SAXS), and reduced histological maturation of the tissue in unloaded compared to loaded tendons. However, by 4 weeks no differences remained. SAXS and histology revealed heterogeneous tissue maturation with more mature tissue at the peripheral region compared to the center of the callus. Thus, mechanical loading advances Achilles tendon biomechanical and collagen properties earlier compared to unloaded tendons, and the spatial variation in tissue maturation and collagen organization across the callus suggests important regional (mechano-) biological activities that require more investigation.
Identifiants
pubmed: 33315857
doi: 10.1371/journal.pone.0236681
pii: PONE-D-20-20121
pmc: PMC7735574
doi:
Substances chimiques
Collagen
9007-34-5
Botulinum Toxins, Type A
EC 3.4.24.69
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0236681Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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