Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
10 Dec 2020
10 Dec 2020
Historique:
received:
04
10
2020
revised:
12
11
2020
accepted:
06
12
2020
pubmed:
15
12
2020
medline:
15
12
2020
entrez:
14
12
2020
Statut:
aheadofprint
Résumé
miR-26b has been implicated in a wide range of human diseases, including cancer, diabetes, heart disease, Alzheimer's disease and osteoarthritis. To provide a tool to explore the importance of miR-26b in this broad context, we have generated and characterized a homozygous miR-26b stem-loop knockout human iPSC line. This gene-edited line exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. This iPSC line will be valuable for studies investigating disease mechanisms and testing therapeutic strategies in vitro.
Identifiants
pubmed: 33316599
pii: S1873-5061(20)30419-0
doi: 10.1016/j.scr.2020.102118
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102118Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.