PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells.
Autophagy
Cell Line, Tumor
Cell Survival
DNA Modification Methylases
/ biosynthesis
DNA Repair Enzymes
/ biosynthesis
Gene Expression Regulation, Neoplastic
Gene Knockout Techniques
Glioblastoma
/ genetics
Guanine Nucleotide Exchange Factors
/ genetics
Humans
Signal Transduction
Tumor Suppressor Proteins
/ biosynthesis
rab GTP-Binding Proteins
/ genetics
rhoA GTP-Binding Protein
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 12 2020
14 12 2020
Historique:
received:
20
05
2020
accepted:
17
11
2020
entrez:
15
12
2020
pubmed:
16
12
2020
medline:
29
4
2021
Statut:
epublish
Résumé
A signalling pathway involving PLEKHG5 (guanine exchange factor) for the Ras superfamily member RAB26 to transcription factor NF-κB was discovered in autophagy. PLEKHG5 was reported in glioblastoma multiforme (GBM) and correlates with patient survival. Thus, the generation of a cellular model for understanding PLEKHG5 signalling is the study purpose. We generated a CRISPR/Cas9-mediated knockout of PLEKHG5 in U251-MG glioblastoma cells and analysed resulting changes. Next, we used a mRFP-GFP-LC3
Identifiants
pubmed: 33318498
doi: 10.1038/s41598-020-77958-3
pii: 10.1038/s41598-020-77958-3
pmc: PMC7736842
doi:
Substances chimiques
Guanine Nucleotide Exchange Factors
0
PLEKHG5 protein, human
0
Tumor Suppressor Proteins
0
RHOA protein, human
124671-05-2
DNA Modification Methylases
EC 2.1.1.-
MGMT protein, human
EC 2.1.1.63
Rab26 protein, human
EC 3.6.1.-.
rab GTP-Binding Proteins
EC 3.6.5.2
rhoA GTP-Binding Protein
EC 3.6.5.2
DNA Repair Enzymes
EC 6.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21858Références
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