RNA-Seq transcriptome data of human cells infected with influenza A/Puerto Rico/8/1934 (H1N1) virus.
H1N1
Human cells
Influenza A virus
RNA-seq
Transcriptome
Journal
Data in brief
ISSN: 2352-3409
Titre abrégé: Data Brief
Pays: Netherlands
ID NLM: 101654995
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
08
09
2020
revised:
24
11
2020
accepted:
25
11
2020
entrez:
15
12
2020
pubmed:
16
12
2020
medline:
16
12
2020
Statut:
epublish
Résumé
Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell lines (A549, HEK293FT, WI-38 VA-13) were selected for RNA sequencing using Illumina NextSeq500 platform. Raw data were applied to the bioinformatic pipeline, which includes quality control with FastQC and MultiQC, adapter and quality trimming with Cutadapt, filtering to the genome of influenza A with STAR, transcript quantification with Salmon tool (GRCh38_RefSeq_Transcripts). Differential expressed genes were identified using R package DESeq2 with FDR-adjusted p-value < 0.001 and absolute value of log2(FC) > 1. Lists of differentially expressed genes is provided. The raw and processed RNA-seq data presented in this article were deposited to the European Nucleotide Archive via the ArrayExpress partner repository with the dataset accession number E-MTAB-9511 .
Identifiants
pubmed: 33318985
doi: 10.1016/j.dib.2020.106604
pii: S2352-3409(20)31484-0
pmc: PMC7725734
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106604Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article.
Références
F1000Res. 2015 Dec 30;4:1521
pubmed: 26925227
Bioinformatics. 2016 Oct 1;32(19):3047-8
pubmed: 27312411
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Nat Methods. 2017 Apr;14(4):417-419
pubmed: 28263959
BMC Mol Biol. 2006 Jan 31;7:3
pubmed: 16448564
Genome Biol. 2014;15(12):550
pubmed: 25516281
Nucleic Acids Res. 2019 Jan 8;47(D1):D711-D715
pubmed: 30357387