Impact of Acute HIV Infection and Early Antiretroviral Therapy on the Human Gut Microbiome.
ART
HIV
immune activation
inflammation
microbiome
Journal
Open forum infectious diseases
ISSN: 2328-8957
Titre abrégé: Open Forum Infect Dis
Pays: United States
ID NLM: 101637045
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
17
06
2019
accepted:
19
08
2019
entrez:
16
12
2020
pubmed:
21
8
2019
medline:
21
8
2019
Statut:
epublish
Résumé
Intestinal microbial dysbiosis is evident in chronic HIV-infected individuals and may underlie inflammation that persists even during antiretroviral therapy (ART). It remains unclear, however, how early after HIV infection gut dysbiosis emerges and how it is affected by early ART. Fecal microbiota were studied by 16s rDNA sequencing in 52 Thai men who have sex with men (MSM), at diagnosis of acute HIV infection (AHI), Fiebig Stages 1-5 (F1-5), and after 6 months of ART initiation, and in 7 Thai MSM HIV-uninfected controls. Dysbiotic bacterial taxa were associated with relevant inflammatory markers. Fecal microbiota profiling of AHI pre-ART vs HIV-uninfected controls showed a mild dysbiosis. Transition from F1-3 of acute infection was characterized by enrichment in pro-inflammatory bacteria. Lower proportions of Bacteroidetes and higher frequencies of Proteobacteria and Fusobacteria members were observed post-ART compared with pre-ART. Fusobacteria members were positively correlated with levels of soluble CD14 in AHI post-ART. Evidence of gut dysbiosis was observed during early acute HIV infection and was partially restored upon early ART initiation. The association of dysbiotic bacterial taxa with inflammatory markers suggests that a potential relationship between altered gut microbiota and systemic inflammation may also be established during AHI.
Sections du résumé
Background
Intestinal microbial dysbiosis is evident in chronic HIV-infected individuals and may underlie inflammation that persists even during antiretroviral therapy (ART). It remains unclear, however, how early after HIV infection gut dysbiosis emerges and how it is affected by early ART.
Methods
Fecal microbiota were studied by 16s rDNA sequencing in 52 Thai men who have sex with men (MSM), at diagnosis of acute HIV infection (AHI), Fiebig Stages 1-5 (F1-5), and after 6 months of ART initiation, and in 7 Thai MSM HIV-uninfected controls. Dysbiotic bacterial taxa were associated with relevant inflammatory markers.
Results
Fecal microbiota profiling of AHI pre-ART vs HIV-uninfected controls showed a mild dysbiosis. Transition from F1-3 of acute infection was characterized by enrichment in pro-inflammatory bacteria. Lower proportions of Bacteroidetes and higher frequencies of Proteobacteria and Fusobacteria members were observed post-ART compared with pre-ART. Fusobacteria members were positively correlated with levels of soluble CD14 in AHI post-ART.
Conclusions
Evidence of gut dysbiosis was observed during early acute HIV infection and was partially restored upon early ART initiation. The association of dysbiotic bacterial taxa with inflammatory markers suggests that a potential relationship between altered gut microbiota and systemic inflammation may also be established during AHI.
Identifiants
pubmed: 33324725
doi: 10.1093/ofid/ofz367
pii: ofz367
pmc: PMC7724511
doi:
Types de publication
Journal Article
Langues
eng
Pagination
ofz367Subventions
Organisme : CCR NIH HHS
ID : HHSN261200800001C
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
Investigateurs
Nipat Teeratakulpisarn
(N)
Supanit Pattanachaiwit
(S)
Mark de Souza
(M)
James Fletcher
(J)
Eugene Kroon
(E)
Ponpen Tantivitayakul
(P)
Duanghathai Suttichom
(D)
Somprartthana Rattanamanee
(S)
Kultida Poltavee
(K)
Jintana Intasan
(J)
Tassanee Luekasemsuk
(T)
Hathairat Savadsuk
(H)
Somporn Tipsuk
(S)
Suwanna Puttamsawin
(S)
Khunthalee Benjapornpong
(K)
Nisakorn Ratnaratorn
(N)
Patcharin Eamyoung
(P)
Sasiwimol Ubolyam
(S)
Robert O'Connell
(R)
Siriwat Akapirat
(S)
Bessara Nuntapinit
(B)
Nantana Tantibul
(N)
Nampueng Churikanont
(N)
Saowanit Getchalarat
(S)
Sandhya Vasan
(S)
Rapee Trichavaroj
(R)
Chayada Sajiaweerawan
(C)
Yuwadee Phuang-Ngern
(Y)
Surat Jongrakthaitae
(S)
Suchada Sukhumvittaya
(S)
Putida Saetun
(P)
Weerawan Chuenarom
(W)
Nelson Michael
(N)
Ellen Turk
(E)
Corinne McCullough
(C)
Oratai Butterworth
(O)
Mark Milazzo
(M)
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Published by Oxford University Press on behalf of Infectious Diseases Society of America 2019.
Références
Nat Med. 2006 Dec;12(12):1365-71
pubmed: 17115046
J Infect Dis. 2015 Jan 1;211(1):19-27
pubmed: 25057045
Curr Gastroenterol Rep. 2009 Aug;11(4):307-13
pubmed: 19615307
Mucosal Immunol. 2015 Jul;8(4):760-72
pubmed: 25407519
Clin Lab Med. 2014 Dec;34(4):733-45
pubmed: 25439273
J Infect Dis. 2014 Oct 15;210(8):1228-38
pubmed: 24755434
Mucosal Immunol. 2008 Jan;1(1):23-30
pubmed: 19079157
Mucosal Immunol. 2014 Jul;7(4):983-94
pubmed: 24399150
J Cheminform. 2010 Oct 18;2(1):9
pubmed: 20955607
PLoS Pathog. 2010 Aug 19;6(8):e1001052
pubmed: 20808901
Cytometry A. 2011 Feb;79(2):167-74
pubmed: 21265010
Nat Genet. 2017 Apr;49(4):568-578
pubmed: 28263315
Bioinformatics. 2018 Apr 15;34(8):1411-1413
pubmed: 29028892
J Infect Dis. 2011 Mar 15;203(6):780-90
pubmed: 21252259
Immunol Rev. 2013 Jul;254(1):343-54
pubmed: 23772630
Gastroenterology. 2014 May;146(6):1513-24
pubmed: 24440671
JCI Insight. 2016 Jul 7;1(10):
pubmed: 27446990
Curr HIV/AIDS Rep. 2012 Mar;9(1):44-51
pubmed: 22193889
Microbiome. 2013 Oct 12;1(1):26
pubmed: 24451087
PLoS Pathog. 2014 Jan 30;10(1):e1003890
pubmed: 24497828
Immunity. 2010 Jun 25;32(6):737-42
pubmed: 20620940
EBioMedicine. 2016 Jan 28;5:135-46
pubmed: 27077120
Sci Transl Med. 2013 Jul 10;5(193):193ra91
pubmed: 23843452
AIDS. 2015 Apr 24;29(7):793-800
pubmed: 25985402
J Infect Dis. 2016 Oct 1;214 Suppl 2:S58-66
pubmed: 27625432
Cell Host Microbe. 2016 Mar 9;19(3):311-22
pubmed: 26962942
PLoS Pathog. 2014 Feb 20;10(2):e1003829
pubmed: 24586144
Cell Host Microbe. 2013 Sep 11;14(3):329-39
pubmed: 24034618
Blood. 2008 Oct 1;112(7):2826-35
pubmed: 18664624
Int J Tryptophan Res. 2009;2:1-19
pubmed: 22084578
Clin Infect Dis. 2017 Jan 15;64(2):124-131
pubmed: 27737952
Cell Metab. 2016 Jul 12;24(1):41-50
pubmed: 27320064
Curr Opin HIV AIDS. 2018 Jan;13(1):15-21
pubmed: 29028666
AIDS Rev. 2008 Jan-Mar;10(1):36-46
pubmed: 18385779
J Infect Dis. 2014 Mar;209(6):931-9
pubmed: 24185941
Mucosal Immunol. 2008 Jul;1(4):279-88
pubmed: 19079189
J Immunol. 2008 Nov 15;181(10):6687-91
pubmed: 18981083
Cancer Res. 2014 Aug 15;74(16):4217-21
pubmed: 25074615
Nat Med. 2018 Sep;24(9):1313-1316
pubmed: 30061696
PLoS Pathog. 2014 Dec 11;10(12):e1004543
pubmed: 25503054