3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.
Animals
Cell Membrane
/ drug effects
Cell Nucleus
/ drug effects
Cells, Cultured
Glucose
/ pharmacology
Imaging, Three-Dimensional
Insulin
/ metabolism
Insulin-Secreting Cells
/ drug effects
Mice, Inbred C57BL
Microscopy, Electron, Scanning
Microtubules
/ drug effects
Organelles
/ metabolism
Secretory Vesicles
/ drug effects
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 02 2021
01 02 2021
Historique:
received:
07
10
2020
revised:
14
11
2020
accepted:
18
11
2020
entrez:
16
12
2020
pubmed:
17
12
2020
medline:
2
9
2021
Statut:
ppublish
Résumé
Microtubules play a major role in intracellular trafficking of vesicles in endocrine cells. Detailed knowledge of microtubule organization and their relation to other cell constituents is crucial for understanding cell function. However, their role in insulin transport and secretion is under debate. Here, we use FIB-SEM to image islet β cells in their entirety with unprecedented resolution. We reconstruct mitochondria, Golgi apparati, centrioles, insulin secretory granules, and microtubules of seven β cells, and generate a comprehensive spatial map of microtubule-organelle interactions. We find that microtubules form nonradial networks that are predominantly not connected to either centrioles or endomembranes. Microtubule number and length, but not microtubule polymer density, vary with glucose stimulation. Furthermore, insulin secretory granules are enriched near the plasma membrane, where they associate with microtubules. In summary, we provide the first 3D reconstructions of complete microtubule networks in primary mammalian cells together with evidence regarding their importance for insulin secretory granule positioning and thus their supportive role in insulin secretion.
Identifiants
pubmed: 33326005
pii: 211599
doi: 10.1083/jcb.202010039
pmc: PMC7748794
pii:
doi:
Substances chimiques
Insulin
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Müller et al.
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