An Acoustic Platform for Single-Cell, High-Throughput Measurements of the Viscoelastic Properties of Cells.
Piezo1
acoustic
cell mechanics
elasticity
mechanobiology
microfluidics
viscoelasticity
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
15
09
2020
revised:
01
12
2020
pubmed:
17
12
2020
medline:
25
6
2021
entrez:
16
12
2020
Statut:
ppublish
Résumé
Cellular processes including adhesion, migration, and differentiation are governed by the distinct mechanical properties of each cell. Importantly, the mechanical properties of individual cells can vary depending on local physical and biochemical cues in a time-dependent manner resulting in significant inter-cell heterogeneity. While several different methods have been developed to interrogate the mechanical properties of single cells, throughput to capture this heterogeneity remains an issue. Here, single-cell, high-throughput characterization of adherent cells is demonstrated using acoustic force spectroscopy (AFS). AFS works by simultaneously, acoustically driving tens to hundreds of silica beads attached to cells away from the cell surface, allowing the user to measure the stiffness of adherent cells under multiple experimental conditions. It is shown that cells undergo marked changes in viscoelasticity as a function of temperature, by altering the temperature within the AFS microfluidic circuit between 21 and 37 °C. In addition, quantitative differences in cells exposed to different pharmacological treatments specifically targeting the membrane-cytoskeleton interface are shown. Further, the high-throughput format of the AFS is utilized to rapidly probe, in excess of 1000 cells, three different cell lines expressing different levels of a mechanosensitive protein, Piezo1, demonstrating the ability to differentiate between cells based on protein expression levels.
Identifiants
pubmed: 33326190
doi: 10.1002/smll.202005759
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2005759Informations de copyright
© 2020 Wiley-VCH GmbH.
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