A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge.
Animals
Antibodies, Viral
/ immunology
Antigens, Viral
/ immunology
Body Weight
COVID-19
/ immunology
COVID-19 Vaccines
/ immunology
Cell Line
Cricetinae
Disease Models, Animal
Dose-Response Relationship, Immunologic
Genome, Viral
Lung
/ pathology
Mice, Inbred C57BL
Mutation
/ genetics
SARS-CoV-2
/ genetics
Spike Glycoprotein, Coronavirus
/ genetics
Vaccination
Vaccines, Synthetic
/ immunology
Vesicular stomatitis Indiana virus
/ immunology
Viral Load
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
16 12 2020
16 12 2020
Historique:
received:
18
06
2020
accepted:
17
11
2020
entrez:
17
12
2020
pubmed:
18
12
2020
medline:
23
12
2020
Statut:
epublish
Résumé
The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-∆G-spike vaccine, in which the glycoprotein of VSV is replaced by the spike protein of SARS-CoV-2. In-vitro characterization of this vaccine indicates the expression and presentation of the spike protein on the viral membrane with antigenic similarity to SARS-CoV-2. A golden Syrian hamster in-vivo model for COVID-19 is implemented. We show that a single-dose vaccination results in a rapid and potent induction of SARS-CoV-2 neutralizing antibodies. Importantly, vaccination protects hamsters against SARS-CoV-2 challenge, as demonstrated by the abrogation of body weight loss, and alleviation of the extensive tissue damage and viral loads in lungs and nasal turbinates. Taken together, we suggest the recombinant VSV-∆G-spike as a safe, efficacious and protective vaccine against SARS-CoV-2.
Identifiants
pubmed: 33328475
doi: 10.1038/s41467-020-20228-7
pii: 10.1038/s41467-020-20228-7
pmc: PMC7745033
doi:
Substances chimiques
Antibodies, Viral
0
Antigens, Viral
0
COVID-19 Vaccines
0
Spike Glycoprotein, Coronavirus
0
Vaccines, Synthetic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6402Références
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