Exosomal MiR-769-5p Exacerbates Ultraviolet-Induced Bystander Effect by Targeting

TGFBR1 exosomes miRNA photo damage ultraviolet–radiation induced bystander effects

Journal

Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006

Informations de publication

Date de publication:
2020
Historique:
received: 05 09 2020
accepted: 28 10 2020
entrez: 17 12 2020
pubmed: 18 12 2020
medline: 18 12 2020
Statut: epublish

Résumé

Exosomal microRNAs have been investigated in bystander effect, but it is unclear whether microRNA works in ultraviolet radiation-induced bystander effects (UV-RIBEs) and what the underlying mechanism could be. Exosomes from ultraviolet (UV)-irradiated human skin fibroblasts (HSFs) were isolated and transferred to normal HSFs, followed by the detection of proliferation rate, oxidative damage level, and apoptosis rate. Exosomal miRNAs were evaluated and screened with miRNA sequencing and quantitative reverse transcriptase-polymerase chain reaction method. MiRNA shuttle and bystander photodamage reactions were observed after transfection of miR-769-5p. MiR-769-5p targeting gene transforming growth factor-β1 (

Identifiants

pubmed: 33329055
doi: 10.3389/fphys.2020.603081
pmc: PMC7719707
doi:

Types de publication

Journal Article

Langues

eng

Pagination

603081

Informations de copyright

Copyright © 2020 Ni, Ma, Tao, Liu, Hua, Cheng, Wang, Zhou and Luo.

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Auteurs

Na Ni (N)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Weiwei Ma (W)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Yanling Tao (Y)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Juan Liu (J)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Hui Hua (H)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Jiawei Cheng (J)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Jie Wang (J)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Bingrong Zhou (B)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Dan Luo (D)

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Classifications MeSH