Identification of a Stable Hydrogen-Driven Microbiome in a Highly Radioactive Storage Facility on the Sellafield Site.
Hydrogenophaga
Sellafield
hydrogen metabolism
microbial ecology
spent nuclear fuel
spent nuclear fuel ponds
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
26
07
2020
accepted:
26
10
2020
entrez:
17
12
2020
pubmed:
18
12
2020
medline:
18
12
2020
Statut:
epublish
Résumé
The use of nuclear power has been a significant part of the United Kingdom's energy portfolio with the Sellafield site being used for power production and more recently reprocessing and decommissioning of spent nuclear fuel activities. Before being reprocessed, spent nuclear fuel is stored in water ponds with significant levels of background radioactivity and in high alkalinity (to minimize fuel corrosion). Despite these challenging conditions, the presence of microbial communities has been detected. To gain further insight into the microbial communities present in extreme environments, an indoor, hyper-alkaline, oligotrophic, and radioactive spent fuel storage pond (INP) located on the Sellafield site was analyzed. Water samples were collected from sample points within the INP complex, and also the purge water feeding tank (FT) that supplies water to the pond, and were screened for the presence of the 16S and 18S rRNA genes to inform sequencing requirements over a period of 30 months. Only 16S rRNA genes were successfully amplified for sequencing, suggesting that the microbial communities in the INP were dominated by prokaryotes. Quantitative Polymerase Chain Reaction (qPCR) analysis targeting 16S rRNA genes suggested that bacterial cells in the order of 10
Identifiants
pubmed: 33329459
doi: 10.3389/fmicb.2020.587556
pmc: PMC7732693
doi:
Types de publication
Journal Article
Langues
eng
Pagination
587556Informations de copyright
Copyright © 2020 Ruiz-Lopez, Foster, Boothman, Cole, Morris and Lloyd.
Références
Int J Syst Bacteriol. 1994 Jul;44(3):427-34
pubmed: 7520734
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Front Bioeng Biotechnol. 2016 Jan 20;4:4
pubmed: 26835451
Curr Opin Biotechnol. 2005 Jun;16(3):254-60
pubmed: 15916892
Faraday Discuss. 2015;180:301-11
pubmed: 25932469
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
PLoS One. 2018 Oct 4;13(10):e0205228
pubmed: 30286173
J Ind Microbiol. 1995 Feb;14(2):76-84
pubmed: 7766213
Appl Environ Microbiol. 2013 Sep;79(17):5112-20
pubmed: 23793624
Appl Environ Microbiol. 2002 Feb;68(2):699-704
pubmed: 11823209
Appl Environ Microbiol. 2004 Apr;70(4):1944-55
pubmed: 15066784
FEMS Microbiol Lett. 2004 Nov 15;240(2):155-62
pubmed: 15522503
J Appl Microbiol. 2011 Aug;111(2):338-49
pubmed: 21624021
Environ Sci Technol. 2007 Oct 15;41(20):7087-93
pubmed: 17993152
Bioresour Technol. 2009 Mar;100(6):1915-21
pubmed: 19054668
PLoS One. 2013;8(2):e57923
pubmed: 23460914
PLoS One. 2015 Apr 27;10(4):e0124863
pubmed: 25915866
Curr Protoc Mol Biol. 2001 May;Chapter 7:Unit7.4A
pubmed: 18265267
ACS Omega. 2019 Aug 26;4(11):14420-14429
pubmed: 31528795
Sci Total Environ. 2020 Jun 10;720:137515
pubmed: 32325573
J Vis Exp. 2016 Apr 16;(110):
pubmed: 27167458
Mikrobiologiia. 2009 Jan-Feb;78(1):106-16
pubmed: 19334603
Curr Microbiol. 1998 Dec;37(6):387-94
pubmed: 9806976
FEMS Microbiol Lett. 2014 Oct;359(1):81-4
pubmed: 25091383
FEMS Microbiol Lett. 2008 Jan;278(1):94-100
pubmed: 18031533
Nat Commun. 2014 May 21;5:3900
pubmed: 24845058
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):163-168
pubmed: 22368173
Environ Sci Process Impacts. 2018 Jun 20;20(6):956-964
pubmed: 29774347
J Bacteriol. 2007 Dec;189(23):8746-9
pubmed: 17905994
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685-9
pubmed: 1608980
J Phycol. 2016 Oct;52(5):689-703
pubmed: 27470701
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2335-2341
pubmed: 15545480
Bioresour Technol. 2014 Nov;172:449-452
pubmed: 25262456
ISME J. 2012 Aug;6(8):1621-4
pubmed: 22402401
mBio. 2018 Jun 26;9(3):
pubmed: 29946053
Appl Environ Microbiol. 1992 Mar;58(3):1019-23
pubmed: 1575473
Stand Genomic Sci. 2017 May 10;12:32
pubmed: 28496940
Front Microbiol. 2018 Mar 09;9:377
pubmed: 29593667
Int J Syst Bacteriol. 1993 Jan;43(1):125-34
pubmed: 8427804
J Hazard Mater. 2008 Feb 28;151(1):185-93
pubmed: 17611021
J Appl Microbiol. 2005;98(3):727-40
pubmed: 15715877
Lett Appl Microbiol. 2008 Jul;47(1):60-6
pubmed: 18544140
Nat Methods. 2013 Oct;10(10):996-8
pubmed: 23955772
Science. 1992 Aug 7;257(5071):782-4
pubmed: 1496397
World J Microbiol Biotechnol. 2018 Jun 9;34(7):89
pubmed: 29886519
Appl Environ Microbiol. 2012 Jun;78(12):4481-9
pubmed: 22492459
J Vis Exp. 2012 May 22;(63):e3998
pubmed: 22664923
J Eukaryot Microbiol. 1999 Jul-Aug;46(4):327-38
pubmed: 10461381
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):648-653
pubmed: 22544791
J Contam Hydrol. 2008 Dec 12;102(3-4):285-95
pubmed: 19008016
Chemosphere. 2012 Oct;89(6):764-8
pubmed: 22850277
Res Microbiol. 1997 Jul-Aug;148(6):526-8
pubmed: 9765835
Genome Announc. 2016 Jan 14;4(1):
pubmed: 26769923
J Ind Microbiol Biotechnol. 2005 Apr;32(4):155-62
pubmed: 15778866
BMC Bioinformatics. 2012 Feb 14;13:31
pubmed: 22333067
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1328-1333
pubmed: 24425738
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22
pubmed: 20534432
J Gen Appl Microbiol. 2010 Jun;56(3):267-71
pubmed: 20647684
Antonie Van Leeuwenhoek. 2015 Sep;108(3):711-20
pubmed: 26155772
Appl Environ Microbiol. 1984 Jul;48(1):81-7
pubmed: 6433795
Appl Environ Microbiol. 2004 Dec;70(12):7378-87
pubmed: 15574939
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2231-2235
pubmed: 15545463
J Chem Technol Biotechnol. 1990;49(4):331-43
pubmed: 1366965
Int J Syst Evol Microbiol. 2003 May;53(Pt 3):669-673
pubmed: 12807184
Int Microbiol. 2005 Sep;8(3):223-30
pubmed: 16200501
Front Microbiol. 2020 Apr 07;11:515
pubmed: 32318035
Biofouling. 2004 Feb;20(1):35-42
pubmed: 15079891
Appl Environ Microbiol. 2002 Apr;68(4):1548-55
pubmed: 11916667
J Basic Microbiol. 2006;46(2):94-107
pubmed: 16598832
Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):341-344
pubmed: 15653898
Int J Syst Evol Microbiol. 2006 Dec;56(Pt 12):2819-2823
pubmed: 17158982
Prikl Biokhim Mikrobiol. 2013 May-Jun;49(3):249-54
pubmed: 23882943
Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1491-1498
pubmed: 11491350
Naturwissenschaften. 2002 Aug;89(8):366-70
pubmed: 12435038
BMC Bioinformatics. 2004 Aug 19;5:113
pubmed: 15318951
Appl Environ Microbiol. 2006 Sep;72(9):6288-98
pubmed: 16957256
Int J Syst Evol Microbiol. 2009 Jul;59(Pt 7):1630-4
pubmed: 19542147
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2191-4
pubmed: 18768628
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955-9
pubmed: 2413450
Nat Biotechnol. 1998 Jun;16(6):572-5
pubmed: 9624690
BMC Res Notes. 2017 Jun 14;10(1):212
pubmed: 28615078
Int J Syst Evol Microbiol. 2012 Jan;62(Pt 1):106-111
pubmed: 21335496
Int J Syst Bacteriol. 1991 Apr;41(2):324-5
pubmed: 1854644
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
FEMS Microbiol Rev. 2003 Jun;27(2-3):411-25
pubmed: 12829277
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
BMC Genomics. 2009 Sep 22;10:447
pubmed: 19772637
Appl Microbiol Biotechnol. 2004 Jan;63(4):466-73
pubmed: 12682791
J Ind Microbiol Biotechnol. 2001 Jun;26(6):333-40
pubmed: 11571615
Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):2997-3002
pubmed: 22307515
Environ Sci Pollut Res Int. 2018 Jul;25(21):20518-20526
pubmed: 29063404
Int J Syst Evol Microbiol. 2016 Jan;66(1):385-390
pubmed: 26530339
Biofouling. 2017 Feb;33(2):195-209
pubmed: 28198664
Int J Syst Evol Microbiol. 2014 May;64(Pt 5):1697-1702
pubmed: 24523446
Int J Environ Res Public Health. 2014 Jun 10;11(6):6099-118
pubmed: 24919131
Acta Microbiol Immunol Hung. 2011 Dec;58(4):371-82
pubmed: 22207294
Appl Radiat Isot. 2012 Jul;70(7):1162-5
pubmed: 22698817
Int J Syst Bacteriol. 1997 Apr;47(2):408-13
pubmed: 9103629
J Bacteriol. 1974 Sep;119(3):736-47
pubmed: 4604283
Bioinformatics. 2011 Aug 15;27(16):2194-200
pubmed: 21700674
Appl Radiat Isot. 2014 Dec;94:254-259
pubmed: 25244071