The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region.

RNAi-based vaccination ambisense RNA cell-to-cell movement protein double-stranded rnas nucleocapsid protein orthotospovirus

Journal

Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200

Informations de publication

Date de publication:
2020
Historique:
received: 07 02 2020
accepted: 09 11 2020
entrez: 17 12 2020
pubmed: 18 12 2020
medline: 18 12 2020
Statut: epublish

Résumé

Tomato spotted wilt virus (TSWV) is a devastating plant pathogen, causing huge crop losses worldwide. Unfortunately, due to its wide host range and emergence of resistance breaking strains, its management is challenging. Up to now, resistance to TSWV infection based on RNA interference (RNAi) has been achieved only in transgenic plants expressing parts of the viral genome or artificial microRNAs targeting it. Exogenous application of double-stranded RNAs (dsRNAs) for inducing virus resistance in plants, namely RNAi-based vaccination, represents an attractive and promising alternative, already shown to be effective against different positive-sense RNA viruses and viroids. In the present study, the protection efficacy of exogenous application of dsRNAs targeting the nucleocapsid (

Identifiants

pubmed: 33329620
doi: 10.3389/fpls.2020.533338
pmc: PMC7732615
doi:

Types de publication

Journal Article

Langues

eng

Pagination

533338

Informations de copyright

Copyright © 2020 Tabein, Jansen, Noris, Vaira, Marian, Behjatnia, Accotto and Miozzi.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Saeid Tabein (S)

Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.
Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.

Marco Jansen (M)

Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.
Laboratory of Virology, Department of Plant Sciences, Wageningen University & Research, Wageningen, Netherlands.

Emanuela Noris (E)

Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.

Anna Maria Vaira (AM)

Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.

Daniele Marian (D)

Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.

S Ali Akbar Behjatnia (SAA)

Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.

Gian Paolo Accotto (GP)

Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.

Laura Miozzi (L)

Institute for Sustainable Plant Protection, National Research Council of Italy, Turin, Italy.

Classifications MeSH