Pre-T cell receptors topologically sample self-ligands during thymocyte β-selection.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
08 01 2021
Historique:
received: 30 07 2020
accepted: 03 12 2020
pubmed: 19 12 2020
medline: 17 2 2021
entrez: 18 12 2020
Statut: ppublish

Résumé

Self-discrimination, a critical but ill-defined molecular process programmed during thymocyte development, requires myriad pre-T cell receptors (preTCRs) and αβTCRs. Using x-ray crystallography, we show how a preTCR applies the concave β-sheet surface of its single variable domain (Vβ) to "horizontally" grab the protruding MHC α2-helix. By contrast, αβTCRs purpose all six complementarity-determining region (CDR) loops of their paired VαVβ module to recognize peptides bound to major histocompatibility complex molecules (pMHCs) in "vertical" head-to-head binding. The preTCR topological fit ensures that CDR3β reaches the peptide's featured C-terminal segment for pMHC sampling, establishing the subsequent αβTCR canonical docking mode. "Horizontal" docking precludes germline CDR1β- and CDR2β-MHC binding to broaden β-chain repertoire diversification before αβTCR-mediated selection refinement. Thus, one subunit successively attunes the recognition logic of related multicomponent receptors.

Identifiants

pubmed: 33335016
pii: science.abe0918
doi: 10.1126/science.abe0918
pmc: PMC8011828
mid: NIHMS1681834
doi:

Substances chimiques

Ligands 0
Receptors, Antigen, T-Cell, alpha-beta 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

181-185

Subventions

Organisme : NHLBI NIH HHS
ID : T32 HL066987
Pays : United States
Organisme : NIBIB NIH HHS
ID : P41 EB002026
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI037581
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI143565
Pays : United States
Organisme : NIH HHS
ID : S10 OD028526
Pays : United States
Organisme : NIGMS NIH HHS
ID : P01 GM047467
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI136960
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI136301
Pays : United States
Organisme : NIH HHS
ID : S10 OD023513
Pays : United States

Informations de copyright

Copyright © 2021, American Association for the Advancement of Science.

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Auteurs

Xiaolong Li (X)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA. jwang@crystal.harvard.edu ellis_reinherz@dfci.harvard.edu.
Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.

Réka Mizsei (R)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA. jwang@crystal.harvard.edu ellis_reinherz@dfci.harvard.edu.

Kemin Tan (K)

Structural Biology Center, X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Lemont, IL, USA.

Robert J Mallis (RJ)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Dermatology, Harvard Medical School, Boston, MA, USA.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

Jonathan S Duke-Cohan (JS)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.

Aoi Akitsu (A)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.

Paul W Tetteh (PW)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.

Abhinav Dubey (A)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.

Wonmuk Hwang (W)

Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
Department of Materials Science & Engineering, Texas A&M University, College Station, TX, USA.
Department of Physics & Astronomy, Texas A&M University, College Station, TX, USA.
School of Computational Sciences, Korea Institute for Advanced Study, Seoul, Republic of Korea.

Gerhard Wagner (G)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

Matthew J Lang (MJ)

Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA.
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.

Haribabu Arthanari (H)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.

Jia-Huai Wang (JH)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA. jwang@crystal.harvard.edu ellis_reinherz@dfci.harvard.edu.
Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Pediatrics, Harvard Medical School, Boston, MA, USA.

Ellis L Reinherz (EL)

Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA. jwang@crystal.harvard.edu ellis_reinherz@dfci.harvard.edu.
Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.

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