A comparison of exosome purification methods using serum of Marek's disease virus (MDV)-vaccinated and -tumor-bearing chickens.
Animal science
Exosome precipitation
Extracellular vesicle
Hematological system
Immunology
Marek's disease virus
Transcriptomics
Veterinary medicine
Virology
miRNA
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
08
11
2018
revised:
08
01
2019
accepted:
03
12
2020
entrez:
18
12
2020
pubmed:
19
12
2020
medline:
19
12
2020
Statut:
epublish
Résumé
Marek's disease (MD) is an alphaherpesvirus (Marek's disease virus, MDV)-induced pathology of chickens associated with paralysis, immunosuppression, neurological signs, and T-cell lymphomas. MD is controlled in poultry production via live attenuated vaccines. The purpose of the current study was to compare methods for precipitating exosomes from vaccinated and protected chicken sera (VEX) and tumor-bearing chicken sera (TEX) for biomarker analysis of vaccine-induced protection and MD lymphomas respectively. A standard polyethylene glycol (PEG, 8%) method was compared to a commercial reagent (total exosome isolation reagent, TEI) for exosome yield and RNA content. Although exosomes purified by PEG or TEI were comparable in size and morphology, TEI-reagent yielded 3-4-fold greater concentration. Relative expression of 8 out of 10
Identifiants
pubmed: 33336096
doi: 10.1016/j.heliyon.2020.e05669
pii: S2405-8440(20)32512-3
pmc: PMC7734234
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e05669Informations de copyright
© 2020 The Authors.
Références
Viruses. 2014 Mar 21;6(3):1379-94
pubmed: 24662606
J Chromatogr A. 2014 Dec 5;1371:125-35
pubmed: 25458527
J Extracell Vesicles. 2014 Sep 08;3:
pubmed: 25279113
mBio. 2014 Jan 21;5(1):e01060-13
pubmed: 24449754
PLoS Pathog. 2011 Feb;7(2):e1001305
pubmed: 21383974
Clin Biochem. 2014 Sep;47(13-14):1286-92
pubmed: 24956264
Blood. 2007 Jun 1;109(11):4944-51
pubmed: 17327404
Ann Clin Biochem. 2008 May;45(Pt 3):233-5
pubmed: 18482908
Biochim Biophys Acta. 1964 Mar 16;82:463-75
pubmed: 14148814
Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10144-9
pubmed: 21646541
J Virol. 2013 Jan;87(1):80-93
pubmed: 23055556
Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):20025-30
pubmed: 18056809
J Virol. 2005 Sep;79(18):11647-59
pubmed: 16140742
J Extracell Vesicles. 2015 Jul 06;4:27378
pubmed: 26154623
Cancers (Basel). 2016 Dec 10;8(12):
pubmed: 27973407
Biochim Biophys Acta. 1960 Jan 15;37:230-7
pubmed: 13792408
Appl Environ Microbiol. 1988 Aug;54(8):1983-8
pubmed: 2845860
Methods. 2012 Feb;56(2):293-304
pubmed: 22285593
J Immunol Methods. 2001 Jan 1;247(1-2):163-74
pubmed: 11150547
Stem Cell Res. 2010 May;4(3):214-22
pubmed: 20138817
J Virol Methods. 2016 Oct;236:111-116
pubmed: 27448823
Sci Rep. 2016 Apr 12;6:23978
pubmed: 27068479
Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):5003-8
pubmed: 21383194
Biophys J. 2017 Sep 19;113(6):1342-1352
pubmed: 28629620
Virology. 2014 Jan 5;448:55-64
pubmed: 24314636
J Virol Methods. 2019 Jan;263:1-9
pubmed: 30316797
Genome Res. 2010 May;20(5):589-99
pubmed: 20439436
J Biol Chem. 1981 Dec 10;256(23):12108-17
pubmed: 7298647
J Extracell Vesicles. 2016 Feb 15;5:29497
pubmed: 26895490
Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22
pubmed: 18228490
BMC Genomics. 2015 Apr 18;16:311
pubmed: 25896894
Oncogene. 2008 Apr 3;27(15):2128-36
pubmed: 17968323
Oncogene. 2007 Apr 26;26(19):2799-803
pubmed: 17072344
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12885-90
pubmed: 18728182
J Immunol. 2007 Aug 1;179(3):1969-78
pubmed: 17641064
J Virol Methods. 2002 Dec;106(2):167-73
pubmed: 12393147
J Exp Med. 2006 May 15;203(5):1307-17
pubmed: 16651385
Biol Chem. 2013 Oct;394(10):1253-62
pubmed: 23770532
Analyst. 2016 Aug 07;141(15):4640-6
pubmed: 27229443
PLoS One. 2015 Dec 21;10(12):e0145686
pubmed: 26690353
Sci Rep. 2014 Dec 23;4:7532
pubmed: 25532487
Int J Cancer. 2007 Sep 1;121(5):1156-61
pubmed: 17487835
Virology. 2018 Sep;522:1-12
pubmed: 29979959
Clin Biochem. 2014 Jan;47(1-2):135-8
pubmed: 24183884
Lab Chip. 2014 Oct 7;14(19):3773-80
pubmed: 25099143
Nanoscale. 2014 Oct 21;6(20):12056-64
pubmed: 25189198
Methods Cell Biol. 2017;142:119-132
pubmed: 28964331
Oncol Lett. 2016 Jul;12(1):488-494
pubmed: 27347170
Oncogene. 2011 Jan 20;30(3):275-86
pubmed: 20956945
J Extracell Vesicles. 2016 Jun 20;5:31655
pubmed: 27330048
Proteomics Clin Appl. 2010 Jan;4(1):84-96
pubmed: 21137018
Kidney Int. 2012 Nov;82(9):1024-32
pubmed: 22785172