Cell-Derived Nanovesicles as Exosome-Mimetics for Drug Delivery Purposes: Uses and Recommendations.
Animals
Biomarkers
Biomimetics
/ methods
Cell Fractionation
/ methods
Cell Line
Cell-Derived Microparticles
/ metabolism
Cells, Cultured
Chemical Phenomena
Chromatography, Gel
Drug Delivery Systems
/ methods
Exosomes
/ metabolism
Humans
Mice
Nanoparticles
/ metabolism
Pharmaceutical Preparations
/ administration & dosage
Transport Vesicles
/ metabolism
Biomimicry
Drug delivery systems
Exosome-mimetics
Exosomes
Intrinsic targeting
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
18
12
2020
pubmed:
19
12
2020
medline:
31
3
2021
Statut:
ppublish
Résumé
Cell-derived Drug Delivery Systems (DDSs), particularly exosomes, have grown in popularity and have been increasingly explored as novel DDSs, due to their intrinsic targeting capabilities. However, clinical translation of exosomes is impeded by the tedious isolation procedures and poor yield. Cell-derived nanovesicles (CDNs) have recently been produced and proposed as exosome-mimetics. Various methods for producing exosome-mimetics have been developed. In this chapter, we present a simple, efficient, and cost-effective CDNs production method that uses common laboratory equipment (microcentrifuge) and spin cups. Through a series of extrusion and size exclusion steps, CDNs are produced from in vitro cell culture and are found to highly resemble the endogenous exosomes. Thus, we envision that this strategy holds great potential as a viable alternative to exosomes in the development of ideal DDS.
Identifiants
pubmed: 33336276
doi: 10.1007/978-1-0716-0943-9_11
doi:
Substances chimiques
Biomarkers
0
Pharmaceutical Preparations
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
147-170Références
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