CryoEM map of Pseudomonas aeruginosa PilQ enables structural characterization of TsaP.

Cryoelectron Microscopy Cyclic GMP / metabolism Fimbriae Proteins / chemistry Protein Multimerization Pseudomonas aeruginosa
PilQ Pseudomonas aeruginosa TsaP cryoEM secretin surface sensing type IV pilus

Journal

Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697

Informations de publication

Date de publication:
06 05 2021
Historique:
received: 01 09 2020
revised: 22 10 2020
accepted: 24 11 2020
pubmed: 19 12 2020
medline: 30 11 2021
entrez: 18 12 2020
Statut: ppublish

Résumé

The type IV pilus machinery is a multi-protein complex that polymerizes and depolymerizes a pilus fiber used for attachment, twitching motility, phage adsorption, natural competence, protein secretion, and surface-sensing. An outer membrane secretin pore is required for passage of the pilus fiber out of the cell. Herein, the structure of the tetradecameric secretin, PilQ, from the Pseudomonas aeruginosa type IVa pilus system was determined to 4.3 Å and 4.4 Å resolution in the presence and absence of C

Identifiants

DOI: 10.1016/j.str.2020.11.019 PMID: 33338410
pubmed: 33338410
pii: S0969-2126(20)30466-4
doi: 10.1016/j.str.2020.11.019
pii:
doi:

Substances chimiques

pilQ protein, bacteria 0
Fimbriae Proteins 147680-16-8
Cyclic GMP H2D2X058MU

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

457-466.e4

Subventions

Organisme : CIHR
ID : MOP 93585
Pays : Canada

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests None.

Auteurs

Matthew McCallum (M)

Program in Molecular Structure & Function, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.

Stephanie Tammam (S)

Program in Molecular Structure & Function, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.

John L Rubinstein (JL)

Program in Molecular Structure & Function, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada; Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada. Electronic address: john.rubinstein@utoronto.ca.

Lori L Burrows (LL)

Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada. Electronic address: lori.burrows@mcmaster.ca.

P Lynne Howell (PL)

Program in Molecular Structure & Function, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada; Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada. Electronic address: howell@sickkids.ca.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › CryoEM map of Pseudomonas aeruginosa PilQ...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides guanyliques › GMP cyclique › CryoEM map of Pseudomonas aeruginosa PilQ...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de la membrane externe bactérienne › Protéines de fimbriae › CryoEM map of Pseudomonas aeruginosa PilQ...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › CryoEM map of Pseudomonas aeruginosa PilQ...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa › CryoEM map of Pseudomonas aeruginosa PilQ...