Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives.
Alginates
/ chemistry
Cell Culture Techniques
Cell Differentiation
Cell Lineage
Cells, Cultured
Endoderm
/ cytology
Fluorescent Antibody Technique
Gene Expression Regulation, Developmental
Humans
Mechanotransduction, Cellular
Microscopy, Fluorescence
Models, Statistical
Phenotype
Pluripotent Stem Cells
/ physiology
Stem Cell Niche
Time Factors
Tissue Engineering
3D Cell culture
Alginate encapsulation
Alginate stiffness
Biophysical cues
hPSC differentiation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
19
12
2020
pubmed:
20
12
2020
medline:
1
4
2021
Statut:
ppublish
Résumé
Biophysical cues synergize with biochemical cues to drive differentiation of pluripotent stem cells through specific phenotypic trajectory. Tools to manipulate the cell biophysical environment and identify the influence of specific environment perturbation in the presence of combinatorial inputs will be critical to control the development trajectory. Here we describe the procedure to perturb biophysical environment of pluripotent stem cells while maintaining them in 3D culture configuration. We also discuss a high-throughput platform for combinatorial perturbation of the cell microenvironment, and detail a statistical procedure to extract dominant environmental influences.
Identifiants
pubmed: 33340355
doi: 10.1007/978-1-0716-1174-6_6
doi:
Substances chimiques
Alginates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
73-92Références
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