Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis.

Acetates / pharmacology Animals Cadmium / pharmacology Carbocyanines Dimerization Electrophoresis, Capillary Fluorescence Resonance Energy Transfer / instrumentation Metallothionein / chemistry Models, Molecular Protein Conformation Quantum Dots Rabbits Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Static Electricity
Capillary electrophoresis FRET Oligomerization Quantum dots

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Feb 2021
Historique:
received: 19 10 2020
revised: 09 12 2020
accepted: 10 12 2020
pubmed: 20 12 2020
medline: 15 4 2021
entrez: 19 12 2020
Statut: ppublish

Résumé

Herein, we report a new simple and easy-to-use approach for the characterization of protein oligomerization based on fluorescence resonance energy transfer (FRET) and capillary electrophoresis with LED-induced detection. The FRET pair consisted of quantum dots (QDs) used as an emission tunable donor (emission wavelength of 450 nm) and a cyanine dye (Cy3), providing optimal optical properties as an acceptor. Nonoxidative dimerization of mammalian metallothionein (MT) was investigated using the donor and acceptor covalently conjugated to MT. The main functions of MTs within an organism include the transport and storage of essential metal ions and detoxification of toxic ions. Upon storage under aerobic conditions, MTs form dimers (as well as higher oligomers), which may play an essential role as mediators in oxidoreduction signaling pathways. Due to metal bridging by Cd

Identifiants

DOI: 10.1016/j.ijbiomac.2020.12.105 PMID: 33340626
pubmed: 33340626
pii: S0141-8130(20)35246-6
doi: 10.1016/j.ijbiomac.2020.12.105
pii:
doi:

Substances chimiques

Acetates 0
Carbocyanines 0
cyanine dye 3 0
Cadmium 00BH33GNGH
Metallothionein 9038-94-2
cadmium acetate 95KC50Z1L0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

53-60

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest Authors declare no conflict of interest.

Auteurs

Kristyna Pavelicova (K)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.

Lucie Vanickova (L)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.

Yazan Haddad (Y)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.

Lukas Nejdl (L)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.

Jan Zitka (J)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.

Silvia Kociova (S)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.

Filip Mravec (F)

Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic.

Tomas Vaculovic (T)

Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech Republic.

Mirek Macka (M)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic; School of Natural Sciences, Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Private Bag 75, Hobart 7001, Australia.

Marketa Vaculovicova (M)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. Electronic address: marketa.vaculovicova@mendelu.cz.

Vojtech Adam (V)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. Electronic address: vojtech.adam@mendelu.cz.

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Classifications MeSH

questionsmedicales.fr › Lipides › Acides gras › Acides gras volatils › Acétates › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Eucaryotes › Animaux › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Cadmium › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcynes › Carbocyanines › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Métabolisme › Dimérisation › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse capillaire › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Métalloprotéines › Métallothionéine › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Boîtes quantiques › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Metallothionein dimerization evidenced by...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électricité › Électricité statique › Metallothionein dimerization evidenced by...