A Streamlined Approach to Rapidly Detect SARS-CoV-2 Infection Avoiding RNA Extraction: Workflow Validation.
Journal
Disease markers
ISSN: 1875-8630
Titre abrégé: Dis Markers
Pays: United States
ID NLM: 8604127
Informations de publication
Date de publication:
2020
2020
Historique:
received:
05
08
2020
accepted:
28
11
2020
entrez:
21
12
2020
pubmed:
22
12
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has rapidly spread worldwide from the beginning of 2020. The presence of viral RNA in samples by nucleic acid (NA) molecular analysis is the only method available to diagnose COVID-19 disease and to assess patients' viral load. Since the demand for laboratory reagents has increased, there has been a worldwide shortage of RNA extraction kits. We, therefore, developed a fast and cost-effective viral genome isolation method that, combined with quantitative RT-PCR assay, detects SARS-CoV-2 RNA in patient samples. The method relies on the addition of Proteinase K followed by a controlled heat-shock incubation and, then,
Identifiants
pubmed: 33343767
doi: 10.1155/2020/8869424
pmc: PMC7727018
doi:
Substances chimiques
Coronavirus Envelope Proteins
0
RNA, Viral
0
envelope protein, SARS-CoV-2
0
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
8869424Informations de copyright
Copyright © 2020 Catia Mio et al.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
Infez Med. 2020 Ahead of print Jun 1;28(2):174-184
pubmed: 32275259
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Int J Antimicrob Agents. 2020 Mar;55(3):105924
pubmed: 32081636
Dis Markers. 2020 Dec 9;2020:8869424
pubmed: 33343767
J Med Virol. 2020 Jun;92(6):548-551
pubmed: 32096567
J Clin Med. 2020 Apr 24;9(4):
pubmed: 32344679
J Med Virol. 2020 Apr;92(4):441-447
pubmed: 31994742
J Mol Diagn. 2017 Mar;19(2):303-312
pubmed: 28041870
Euro Surveill. 2020 Mar;25(9):
pubmed: 32156330
Ann Hematol. 2016 Apr;95(5):739-44
pubmed: 26931113
Euro Surveill. 2020 Apr;25(14):
pubmed: 32290902
Eur J Nucl Med Mol Imaging. 2020 May;47(5):1275-1280
pubmed: 32107577
J Med Virol. 2020 Jun;92(6):538-539
pubmed: 32096564
Clin Exp Pediatr. 2020 Apr;63(4):119-124
pubmed: 32252141