Development of Fluorescence Polarization Immunoassay for Imidacloprid in Environmental and Agricultural Samples.
fluorescence polarization immunoassay
fluorescent tracers
high throughput detection
imidacloprid
pesticide residue
Journal
Frontiers in chemistry
ISSN: 2296-2646
Titre abrégé: Front Chem
Pays: Switzerland
ID NLM: 101627988
Informations de publication
Date de publication:
2020
2020
Historique:
received:
09
10
2020
accepted:
09
11
2020
entrez:
21
12
2020
pubmed:
22
12
2020
medline:
22
12
2020
Statut:
epublish
Résumé
A fluorescence polarization immunoassay (FPIA) for the determination of imidacloprid (IMI) was developed with advantages of simple operation and short assay time. The haptens of IMI, acetamiprid (ACE), and thiamethoxam (THI) were conjugated with fluorescein isothiocyanate ethylenediamine (EDF) and 4'-Aminomethyl fluorescein (AMF), respectively, to prepare six fluorescence tracers. The conjugation of IMI hapten and EDF (IMI-EDF) was selected to develop the FPIA due to the largest fluorescent polarization value increase in the presence of anti-IMI monoclonal antibody. Under the optimum condition, the limit of detection, 50% inhibition concentration and detection range of the FPIA were 1.7, 4.8, and 1.7-16.3 μg/L, respectively. The cross-reactivities (CRs) with the analogs of IMI were negligible except for imidaclothiz with CR of 79.13%. The average recovery of spiked paddy water, corn and cucumber samples were 82.4-118.5% with the RSDs of 7.0-15.9%, which indicated the FPIA had good accuracy. Thus, the developed FPIA was a potential tool for the rapid and accurate determination of IMI in agricultural and environmental samples.
Identifiants
pubmed: 33344425
doi: 10.3389/fchem.2020.615594
pmc: PMC7738439
doi:
Types de publication
Journal Article
Langues
eng
Pagination
615594Informations de copyright
Copyright © 2020 Zhou, Yang, Tao, Eremin, Hua and Wang.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Pest Manag Sci. 2009 Feb;65(2):122-8
pubmed: 18924117
Environ Sci Pollut Res Int. 2018 Sep;25(26):26617-26624
pubmed: 29998448
J Agric Food Chem. 2003 Mar 26;51(7):1823-30
pubmed: 12643637
Food Chem. 2020 May 1;311:126055
pubmed: 31862564
J Agric Food Chem. 2003 Feb 26;51(5):1107-14
pubmed: 12590442
Anal Bioanal Chem. 2008 Jul;391(5):1499-507
pubmed: 18264817
Food Chem. 2021 Jan 15;335:127609
pubmed: 32739808
J Agric Food Chem. 2002 May 22;50(11):3116-21
pubmed: 12009972
Biosens Bioelectron. 2014 Jun 15;56:231-6
pubmed: 24508546
Talanta. 2013 Nov 15;116:33-8
pubmed: 24148369
Chemosphere. 2020 Sep;254:126837
pubmed: 32339803
J Agric Food Chem. 2001 May;49(5):2159-67
pubmed: 11368571
RSC Adv. 2019 Nov 12;9(63):36825-36830
pubmed: 35539050
Sci Total Environ. 2019 Nov 20;692:1291-1303
pubmed: 31539961
J Chromatogr A. 2003 Jun 27;1003(1-2):189-95
pubmed: 12899308
Pestic Biochem Physiol. 2020 Jun;166:104562
pubmed: 32448417
Anal Chim Acta. 2011 Dec 5;708(1-2):123-9
pubmed: 22093354
Anal Chim Acta. 2009 Apr 20;639(1-2):83-9
pubmed: 19345763
J Agric Food Chem. 2013 Oct 2;61(39):9347-55
pubmed: 24050679
Talanta. 2017 Jan 1;162:495-504
pubmed: 27837862
Anal Chim Acta. 2015 Jun 30;881:82-9
pubmed: 26041523
J Pharm Biomed Anal. 2018 Sep 10;159:326-330
pubmed: 30025297
Biomed Pharmacother. 2018 Jan;97:518-527
pubmed: 29091903
Talanta. 2012 Nov 15;101:85-90
pubmed: 23158295