Optical plasticity of mammalian cells.
biophotonics
light scattering
microscopy
nuclear architecture
optical clearing
photonics
tissue optics
tissue transparency
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
revised:
17
12
2020
received:
13
11
2020
accepted:
18
12
2020
pubmed:
22
12
2020
medline:
13
7
2021
entrez:
21
12
2020
Statut:
ppublish
Résumé
Transparency is widespread in nature, ranging from transparent insect wings to ocular tissues that enable you to read this text, and transparent marine vertebrates. And yet, cells and tissue models in biology are usually strongly light scattering and optically opaque, precluding deep optical microscopy. Here we describe the directed evolution of cultured mammalian cells toward increased transparency. We find that mutations greatly diversify the optical phenotype of Chinese Hamster Ovary cells, a cultured mammalian cell line. Furthermore, only three rounds of high-throughput optical selection and competitive growth are required to yield fit cells with greatly improved transparency. Based on 15 monoclonal cell lines derived from this directed evolution experiment, we find that the evolved transparency frequently goes along with a reduction of nuclear granularity and physiological shifts in gene expression profiles. In the future this optical plasticity of mammalian cells may facilitate genetic clearance of living tissues for in vivo microscopy.
Identifiants
pubmed: 33345429
doi: 10.1002/jbio.202000457
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000457Subventions
Organisme : European Research Council
ID : 853619
Pays : International
Informations de copyright
© 2020 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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