In-Vitro Activity of Nano Fluconazole and Conventional Fluconazole against Clinically Important Dermatophytes.

Dermatophyte Fluconazole Minimum inhibitory concentration Nano-particle

Journal

Iranian journal of public health

ISSN: 2251-6085

Titre abrégé: Iran J Public Health

Pays: Iran

ID NLM: 7505531

Informations de publication

Date de publication:
Oct 2020

Historique:

entrez: 21 12 2020

pubmed: 22 12 2020

medline: 22 12 2020

Statut: ppublish

Résumé

Dermatophytosis is one of the most common fungal infections in humans. Antifungals such as fluconazole are effectively used for treating dermatophytosis; however, drug resistance was observed in many cases. Therefore, a newer treatment strategy is essential. This study (Conducted in the Laboratory of the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran in 2018) evaluated the antifungal susceptibility of nano fluconazole compared to conventional fluconazole on dermatophyte isolates using CLSI M38-A2guidelines. Dermatophyte species isolated from clinical cases of dermatophytosis were identified using PCR sequencing techniques. Zeta potential and size of the nano particles containing fluconazole were measured; scanning electron microscope (SEM) was used to determine nano particle structure. The size of liposomal fluconazole obtained was 88.9 ± 12.14 nm with -20.12 ± 3.8 mV for zeta potential. The encapsulation rate for fluconazole was 75.1± 4.2%. MIC MIC value for nano-fluconazole was lower than conventional fluconazole in all dermatophytes species tested; therefore, nano-fluconazole could inhibit the growth of dermatophytes better than fluconazole at a lower concentration of the drug.

Sections du résumé

BACKGROUND BACKGROUND

METHODS METHODS

This study (Conducted in the Laboratory of the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran in 2018) evaluated the antifungal susceptibility of nano fluconazole compared to conventional fluconazole on dermatophyte isolates using CLSI M38-A2guidelines. Dermatophyte species isolated from clinical cases of dermatophytosis were identified using PCR sequencing techniques. Zeta potential and size of the nano particles containing fluconazole were measured; scanning electron microscope (SEM) was used to determine nano particle structure.

RESULTS RESULTS

The size of liposomal fluconazole obtained was 88.9 ± 12.14 nm with -20.12 ± 3.8 mV for zeta potential. The encapsulation rate for fluconazole was 75.1± 4.2%. MIC

CONCLUSION CONCLUSIONS

MIC value for nano-fluconazole was lower than conventional fluconazole in all dermatophytes species tested; therefore, nano-fluconazole could inhibit the growth of dermatophytes better than fluconazole at a lower concentration of the drug.

Identifiants

DOI: 10.18502/ijph.v49i10.4701 PMID: 33346221 PMC: PMC7719649

pubmed: 33346221

doi: 10.18502/ijph.v49i10.4701

pii: IJPH-49-1970

pmc: PMC7719649

doi:

Types de publication

Journal Article

Langues

eng

Pagination

1970-1976

Informations de copyright

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that there is no conflict of interest.

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