Characterization of an intracellular aspartic protease (PsAPA) from Penicillium sp. XT7 and its application in collagen extraction.

Animals Aspartic Acid Proteases / metabolism Collagen / isolation & purification Hydrogen-Ion Concentration Hydrolysis Intracellular Space / enzymology Penicillium / cytology Saccharomycetales / metabolism Swine Temperature
Characterization Collagen extraction Intracellular aspartic protease Penicillium sp. Yield

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
30 May 2021
Historique:
received: 12 07 2020
revised: 09 11 2020
accepted: 03 12 2020
pubmed: 22 12 2020
medline: 11 3 2021
entrez: 21 12 2020
Statut: ppublish

Résumé

An intracellular aspartic protease, PsAPA, was identified from Penicillium sp. XT7. This protease was belonged to penicillopepsin and was expressed in Pichia pastoris GS115. The recombinant PsAPA had a specific activity of 4289.7 ± 261.7 U/mg. The pH and temperature maxima of the enzyme were 3.0 and 30 °C, respectively. The PsAPA was stable in the pH range from 3.0 to 6.0 and was completely inactivated after incubation at 50 °C for 15 min. Presence of Mn

Identifiants

DOI: 10.1016/j.foodchem.2020.128834 PMID: 33348133
pubmed: 33348133
pii: S0308-8146(20)32696-0
doi: 10.1016/j.foodchem.2020.128834
pii:
doi:

Substances chimiques

Collagen 9007-34-5
Aspartic Acid Proteases EC 3.4.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

128834

Informations de copyright

Copyright © 2020. Published by Elsevier Ltd.

Auteurs

Yujie Guo (Y)

Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Xia Li (X)

Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Wei Jia (W)

Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Feng Huang (F)

Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Yunhe Liu (Y)

Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Chunhui Zhang (C)

Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: zhangchunhui@caas.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Characterization of an intracellular aspartic...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Aspartic acid proteases › Characterization of an intracellular aspartic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Characterization of an intracellular aspartic...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Characterization of an intracellular aspartic...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Characterization of an intracellular aspartic...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Characterization of an intracellular aspartic...
questionsmedicales.fr › Eucaryotes › Champignons › Deuteromycota › Penicillium › Characterization of an intracellular aspartic...
questionsmedicales.fr › Eucaryotes › Champignons › Ascomycota › Saccharomycetales › Characterization of an intracellular aspartic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Characterization of an intracellular aspartic...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Characterization of an intracellular aspartic...