Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.
Amoxicillin
/ chemistry
Animals
Anti-Bacterial Agents
/ chemistry
Chitosan
/ chemistry
Drug Carriers
Drug Compounding
Metal Nanoparticles
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Mice
Microbial Sensitivity Tests
Microspheres
Silver
Skin Diseases, Bacterial
/ drug therapy
Staphylococcal Infections
/ drug therapy
Ag nanoparticles/chitosan hybrid composite spheres
MRSA
Multidrug-resistance
amoxicillin
cytotoxicity.
synergistic anti-
MRSA activity
Journal
Current pharmaceutical biotechnology
ISSN: 1873-4316
Titre abrégé: Curr Pharm Biotechnol
Pays: Netherlands
ID NLM: 100960530
Informations de publication
Date de publication:
2021
2021
Historique:
received:
11
07
2020
revised:
17
10
2020
accepted:
11
11
2020
pubmed:
23
12
2020
medline:
17
6
2021
entrez:
22
12
2020
Statut:
ppublish
Résumé
Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings. Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed. Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres. The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively. All isolates showed resistance to the tested antibiotics except vancomycin. MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.
Sections du résumé
BACKGROUND
BACKGROUND
Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.
OBJECTIVE
OBJECTIVE
Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.
METHODS
METHODS
Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres. The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.
RESULTS
RESULTS
All isolates showed resistance to the tested antibiotics except vancomycin. MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L
CONCLUSION
CONCLUSIONS
The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.
Identifiants
pubmed: 33349214
pii: CPB-EPUB-112575
doi: 10.2174/1389201021666201221143537
doi:
Substances chimiques
Anti-Bacterial Agents
0
Drug Carriers
0
Silver
3M4G523W1G
Amoxicillin
804826J2HU
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
686-696Informations de copyright
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