The effect of the interruption of agitation, temporary cooling, and pneumatic tube transportation on platelet quality during storage for transfusion.
Journal
Transfusion
ISSN: 1537-2995
Titre abrégé: Transfusion
Pays: United States
ID NLM: 0417360
Informations de publication
Date de publication:
17 Dec 2020
17 Dec 2020
Historique:
received:
30
05
2020
revised:
05
10
2020
accepted:
15
11
2020
entrez:
22
12
2020
pubmed:
23
12
2020
medline:
23
12
2020
Statut:
aheadofprint
Résumé
Conditions during blood product storage and transportation should maintain quality. The aim of this in vitro study was to investigate the effect of interruption of agitation, temporary cooling (TC), and pneumatic tube system transportation (PTST) on the aggregation ability (AA) and mitochondrial function (MF) of platelet concentrates (PC). A PC was divided equally into four subunits and then allocated to four test groups. The control group (I) was stored as recommended (continuous agitation, 22 ± 2°C) for 4 days. The test groups were stored without agitation (II), stored as recommended, albeit 4°C for 60 minutes on day (d)2 (III) and PTST (IV). Aggregometry was measured using Multiplate (RocheAG; ADPtest, ASPItest, TRAPtest, COLtest) and MF using Oxygraph-2k (Oroboros Instruments). The basal and maximum mitochondrial respiratory rate (MMRR) were determined. AA and MF were measured daily in I and II and AA in III and IV on d2 after TC/PTST. Statistical analysis was performed using tests for matched observations. Eleven PCs were used. TRAP-6 induced AA was significantly lower in II when compared to I on d4 (P = 0.015*). In III the ASPItest was significantly lower (P = 0.032*). IV showed no significant differences. The basal and MMRR were significantly reduced over 4 days in I and II (for both rates in both groups: P = <0.0001*). No significant differences occurred on d4 (P = 0.495). Our results indicate that ex vivo AA and MF of PCs are unaffected, even in no-ideal storage and transport circumstances with respect to agitation, temperature, and force.
Sections du résumé
BACKGROUND
BACKGROUND
Conditions during blood product storage and transportation should maintain quality. The aim of this in vitro study was to investigate the effect of interruption of agitation, temporary cooling (TC), and pneumatic tube system transportation (PTST) on the aggregation ability (AA) and mitochondrial function (MF) of platelet concentrates (PC).
STUDY DESIGN AND METHODS
METHODS
A PC was divided equally into four subunits and then allocated to four test groups. The control group (I) was stored as recommended (continuous agitation, 22 ± 2°C) for 4 days. The test groups were stored without agitation (II), stored as recommended, albeit 4°C for 60 minutes on day (d)2 (III) and PTST (IV). Aggregometry was measured using Multiplate (RocheAG; ADPtest, ASPItest, TRAPtest, COLtest) and MF using Oxygraph-2k (Oroboros Instruments). The basal and maximum mitochondrial respiratory rate (MMRR) were determined. AA and MF were measured daily in I and II and AA in III and IV on d2 after TC/PTST. Statistical analysis was performed using tests for matched observations.
RESULTS
RESULTS
Eleven PCs were used. TRAP-6 induced AA was significantly lower in II when compared to I on d4 (P = 0.015*). In III the ASPItest was significantly lower (P = 0.032*). IV showed no significant differences. The basal and MMRR were significantly reduced over 4 days in I and II (for both rates in both groups: P = <0.0001*). No significant differences occurred on d4 (P = 0.495).
CONCLUSION
CONCLUSIONS
Our results indicate that ex vivo AA and MF of PCs are unaffected, even in no-ideal storage and transport circumstances with respect to agitation, temperature, and force.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : WOA Institution: Goethe-Universität Frankfurt am Main
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2020 The Authors. Transfusion published by Wiley Periodicals LLC. on behalf of AABB.
Références
Blajchman MA. Platelet transfusions: An historical perspective. Hematology Am Soc Hematol Educ Program. 2008;2008:197.
Murphy S, Gardner FH. Effect of storage temperature on maintenance of platelet viability—deleterious effect of refrigerated storage. N Engl J Med. 1969;280(20):1094–1098.
Murphy S, Sayar SN, Gardner FH. Storage of platelet concentrates at 22 degrees C. Blood. 1970;35(4):549–557.
Wallvik J, Stenke L, Akerblom O. The effect of different agitation modes on platelet metabolism, thromboxane formation, and alpha‐granular release during platelet storage. Transfusion. 1990;30(7):639–643.
Egidi MG, DʼAlessandro A, Mandarello G, et al. Troubleshooting in platelet storage temperature and new perspectives through proteomics. Blood Transfus. 2010;8(suppl 3):s73–s81.
Védy D, Robert D, Canellini G, Waldvogel S, Tissot JD. Bacterial contamination of platelet concentrates: Pathogen detection and inactivation methods. Hematol Rep. 2009;1(1):5.
Food and Drug Administration ‐ Center for Biologics Evaluation and Research. Blood Products Advisory Committee Meeting Issue Summary. Available from: https://www.fda.gov/media/114327/download. Accessed September 23, 2019.
British Committee for Standards in Haematology, Blood Transfusion Task Force. Guidelines for the use of platelet transfusions. Br J Haematol. 2003;122(1):10–23.
Bundesärztekammer. Richtlinie zur Gewinnung von Blut und Blutbestandteilen und zur Anwendung von Blutprodukten (Richtlinie Hämotherapie). [cited 2018 Oct 30]. Available from: http://www.bundesaerztekammer.de/fileadmin/user_upload/downloads/pdf‐Ordner/MuE/Richtlinie_Haemotherapie_2017.pdf.
Sahler J, Grimshaw K, Spinelli SL, Refaai MA, Phipps RP, Blumberg N. Platelet storage and transfusions: New concerns associated with an old therapy. Drug Discov Today Dis Mech. 2011;8(1–2):e9–e14.
Perales Villarroel JP, Figueredo R, Guan Y, et al. Increased platelet storage time is associated with mitochondrial dysfunction and impaired platelet function. J Surg Res. 2013;184(1):422–429.
Tóth O, Calatzis A, Penz S, Losonczy H, Siess W. Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood. Thromb Haemost. 2006;96(6):781–788.
Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen‐Dürr P, Gnaiger E. High‐resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph‐2k. Redl H (Hrsg) Proc.11th Congress of the European Shock Society Vienna, Austria. Bologna: Medimond; 2005. p. 107–111.
Skripchenko A, Myrup A, Thompson‐Montgomery D, Awatefe H, Moroff G, Wagner SJ. Periods without agitation diminish platelet mitochondrial function during storage. Transfusion. 2010;50(2):390–399.
Garcia‐Souza LF, Oliveira MF. Mitochondria: Biological roles in platelet physiology and pathology. Int J Biochem Cell Biol. 2014;50:156–160.
Diab YA, Thomas A, Luban NLC, Wong ECC, Wagner SJ, Levy RJ. Acquired cytochrome C oxidase impairment in apheresis platelets during storage: A possible mechanism for depletion of metabolic adenosine triphosphate. Transfusion. 2012;52(5):1024–1030.
Rinder CS, Mathew JP, Rinder HM, Bonan J, Ault KA, Smith BR. Modulation of platelet surface adhesion receptors during cardiopulmonary bypass. Anesthesiology. 1991;75(4):563–570.
Varghese SJ, Unni MK, Mukundan N, Rai R. Platelet functions in cardiopulmonary bypass surgery. Med J Armed Forces India. 2005;61(4):316–321.
Reddoch KM, Pidcoke HF, Montgomery RK, et al. Hemostatic function of apheresis platelets stored at 4°C and 22°C. Shock. 2014;41(Suppl 1):54–61.
Sandgren P, Larsson S, Wai‐San P, Aspevall‐Diedrich B. The effects of pneumatic tube transport on fresh and stored platelets in additive solution. Blood Transfus. 2014;12(1):85–90.
Lancé MD, Marcus MAE, van Oerle R, Theunissen HMS, Henskens YMC. Platelet concentrate transport in pneumatic tube systems—does it work? Vox Sang. 2012;103(1):79–82.
Roeloffzen WWH, Kluin‐Nelemans HC, Veeger NJGM, Bosman L, de Wolf JTM. Transfused stored platelets have the same haemostatic function as circulating native platelets. Vox Sang. 2010;99(2):123–130.
Vetlesen A, Holme PA, Lyberg T, Kjeldsen‐Kragh J. Recovery, survival, and function of transfused platelets and detection of platelet engraftment after allogeneic stem cell transplantation. Transfusion. 2012;52(6):1321–1332.