CTRP9 alleviates foam cells apoptosis by enhancing cholesterol efflux.
ATP Binding Cassette Transporter 1
/ genetics
Adenylate Kinase
/ metabolism
Adiponectin
/ metabolism
Animals
Apoptosis
Biological Transport
Cholesterol
/ metabolism
Foam Cells
/ cytology
Glycoproteins
/ metabolism
Humans
Hydrolysis
Lipid Metabolism
Lipids
/ biosynthesis
Liver X Receptors
/ metabolism
Mice, Inbred C57BL
PPAR gamma
/ metabolism
Receptors, Adiponectin
/ metabolism
THP-1 Cells
Wnt Signaling Pathway
Apoptosis
CTRP9
Cholesterol efflux
Foam cells
Journal
Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844
Informations de publication
Date de publication:
15 02 2021
15 02 2021
Historique:
received:
01
08
2020
revised:
11
12
2020
accepted:
17
12
2020
pubmed:
23
12
2020
medline:
13
8
2021
entrez:
22
12
2020
Statut:
ppublish
Résumé
The apoptosis of foam cells leads to instability of atherosclerotic plaques. This study was designed to explore the protective role of CTRP9 in foam cell apoptosis. In our experiment, CTRP9 alleviated foam cell apoptosis. Meanwhile, CTRP9 upregulated the expression of proteins important for cholesterol efflux, such as LXRα, CYP27A1, ABCG1 and ABCA1, and improved cholesterol efflux in foam cells. Moreover, CTRP9 inhibited Wnt3a and β-catenin expression and β-catenin nuclear translocation in foam cells. In addition, adenovirus overexpression of Wnt3a abolished the effect of CTRP9 on macrophage apoptosis. Mechanistically, the AMPK inhibitor abolished the effect of CTRP9 on foam cell apoptosis, and downregulation of AdipoR1 by siRNA abrogated the activation of AMPK and the effect of CTRP9 on foam cell apoptosis. We concluded that CTRP9 achieved these protective effects on foam cells through the AdipoR1/AMPK pathway.
Identifiants
pubmed: 33352225
pii: S0303-7207(20)30440-8
doi: 10.1016/j.mce.2020.111138
pii:
doi:
Substances chimiques
ATP Binding Cassette Transporter 1
0
Adiponectin
0
CTRP9 protein, mouse
0
Glycoproteins
0
Lipids
0
Liver X Receptors
0
PPAR gamma
0
Receptors, Adiponectin
0
Cholesterol
97C5T2UQ7J
Adenylate Kinase
EC 2.7.4.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111138Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.