A Comparative Study on Phenolic Content, Antioxidant Activity and Anti-Inflammatory Capacity of Aqueous and Ethanolic Extracts of Sorghum in Lipopolysaccharide-Induced RAW 264.7 Macrophages.
RAW 264.7
anti-inflammatory
antioxidant
extraction
phenolics
sorghum
tannins
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
18 Dec 2020
18 Dec 2020
Historique:
received:
02
12
2020
revised:
14
12
2020
accepted:
15
12
2020
entrez:
23
12
2020
pubmed:
24
12
2020
medline:
24
12
2020
Statut:
epublish
Résumé
Sorghum is an important cereal with diverse phenolic compounds that have potential health promoting benefits. The current study comparatively characterized the phenolic contents of two novel black-seeded sorghum lines (SC84 and PI570481) using different extraction systems (water, ethanol and their acidified counterparts) and evaluated their antioxidant and anti-inflammatory activities. Phenolic compositions were determined by spectrophotometric assays and HPLC analysis. Antioxidant activities were assessed by radical scavenging effects on nitric oxide (NO) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, and the oxygen radical absorbance capacity (ORAC). Anti-inflammatory capacity was estimated by measuring levels of pro-inflammatory markers produced by lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Results showed that effects of solvent types and HCl on extraction efficiency differed among phenolic compounds and sorghum samples. Tannins were the most dominant polyphenols in the studied extracts (11.11-136.11 mg epicatechin equivalent/g sorghum). Sorghum extracts exerted more potent scavenging activity on DPPH than NO radicals. In LPS-activated RAW264.7 cells, sorghum extracts dose-dependently inhibited the production of NO, interleukin-6 (IL-6), and intracellular reactive oxygen species (ROS), with ethanolic extracts showing greater anti-inflammatory activity. Positive correlations were noted between tannin content and DPPH radical scavenging activity, and anti-inflammatory capacity. These results suggest the potential role of tannin-rich sorghum extracts against inflammation and associated diseases.
Identifiants
pubmed: 33353009
pii: antiox9121297
doi: 10.3390/antiox9121297
pmc: PMC7767246
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : U.S. Department of Agriculture
ID : ARS-NACA 58-3020-0-023
Références
J Med Food. 2010 Aug;13(4):879-87
pubmed: 20673059
Oxid Med Cell Longev. 2020 Oct 2;2020:2890536
pubmed: 33082907
Food Chem. 2018 Feb 1;240:1121-1130
pubmed: 28946233
Nutr Res Pract. 2016 Jun;10(3):251-8
pubmed: 27247720
J Agric Food Chem. 2004 Jul 14;52(14):4388-94
pubmed: 15237941
Res Immunol. 1991 Sep;142(7):565-9; discussion 596-8
pubmed: 1812549
J Agric Food Chem. 2005 Aug 24;53(17):6813-8
pubmed: 16104804
J Agric Food Chem. 2014 Apr 9;62(14):3150-3159
pubmed: 24655033
J Agric Food Chem. 2010 Aug 25;58(16):9077-82
pubmed: 20681551
J Sep Sci. 2007 Dec;30(18):3268-95
pubmed: 18069740
Molecules. 2010 Oct 21;15(10):7313-52
pubmed: 20966876
Heliyon. 2019 May 07;5(5):e01589
pubmed: 31111105
Molecules. 2013 Feb 19;18(2):2328-75
pubmed: 23429347
J Med Food. 2011 Jul-Aug;14(7-8):799-807
pubmed: 21612457
J Agric Food Chem. 2009 Mar 11;57(5):1797-804
pubmed: 19256554
FEMS Microbiol Lett. 2020 Apr 1;367(7):
pubmed: 32188994
J Biochem Biophys Methods. 2005 Dec 31;65(2-3):45-80
pubmed: 16297980
Nature. 2006 Dec 14;444(7121):860-7
pubmed: 17167474
Free Radic Biol Med. 1993 Mar;14(3):303-11
pubmed: 8458588
Food Chem. 2011 Sep 1;128(1):173-9
pubmed: 25214345
Crit Rev Food Sci Nutr. 2017 Jan 22;57(2):372-390
pubmed: 25875451
Nature. 2008 Jul 24;454(7203):428-35
pubmed: 18650913
Molecules. 2018 May 17;23(5):
pubmed: 29772811
Compr Rev Food Sci Food Saf. 2019 Nov;18(6):2025-2046
pubmed: 33336966
J Diabetes Complications. 2016 Jul;30(5):880-6
pubmed: 27037039
Food Chem. 2015 Feb 1;168:512-9
pubmed: 25172742
J Sci Food Agric. 2014 Aug;94(10):2133-6
pubmed: 24343522
Food Funct. 2017 Dec 13;8(12):4449-4458
pubmed: 29090713
World J Cardiol. 2011 Jan 26;3(1):18-24
pubmed: 21286214
BMC Res Notes. 2015 Sep 01;8:396
pubmed: 26323940
Food Chem. 2015 Aug 1;180:116-123
pubmed: 25766808
J Agric Food Chem. 1998 May;46(5):1887-1892
pubmed: 29072454
Life Sci. 2006 May 15;78(25):2872-88
pubmed: 16325868
J Agric Food Chem. 2010 Oct 27;58(20):10812-6
pubmed: 20919719
Int J Cancer. 2007 Dec 1;121(11):2381-6
pubmed: 17893868
Mutat Res. 1994 Mar 1;305(2):253-64
pubmed: 7510036
Food Funct. 2016 Aug 10;7(8):3410-20
pubmed: 27406291
J Agric Food Chem. 2015 Apr 1;63(12):3205-18
pubmed: 25760759
J Med Food. 2008 Jun;11(2):307-12
pubmed: 18598174
Plant Foods Hum Nutr. 2019 Sep;74(3):307-315
pubmed: 31104201
Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10281-6
pubmed: 22699509
J Med Food. 2018 Oct;21(10):990-998
pubmed: 29733262
Mutat Res. 2008 Jul-Aug;659(1-2):15-30
pubmed: 18485806
Cell Death Dis. 2016 Mar 31;7:e2167
pubmed: 27031964