CSF Levels of Elongation Factor Tu Is Associated With Increased Mortality in Malawian Adults With
Elongation factor Tu (EF-Tu)
HIV—human immunodeficiency virus
Streptococcus pneumoniae
cerebrospinal fluid
meningitis
mortality
proteomics
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
09
2020
accepted:
10
11
2020
entrez:
28
12
2020
pubmed:
29
12
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Mortality from bacterial meningitis, predominately caused by CSF proteomes were analyzed by quantitative Mass-Spectrometry. Spectra were identified using the Swissprot human and TIGR4 pneumococcal protein libraries. Proteins were quantitated and analyzed against mortality. Unique proteins in PM were identified against published normal CSF proteome. Random-Forest models were used to test for protein signatures discriminating outcome. Proteins of interest were tested for their effects on growth and neutrophil opsonophagocytic killing of CSF proteomes were available for 57 Adults with PM (median age 32 years, 60% male, 70% HIV-1 co-infected, mortality 63%). Three hundred sixty individual human and 23 pneumococcal proteins were identified. Of the human protein hits, 30% were not expressed in normal CSF, and these were strongly associated with inflammation and primarily related to neutrophil activity. No human protein signature predicted outcome. However, expression of the essential Excessive
Sections du résumé
Background
Mortality from bacterial meningitis, predominately caused by
Materials/Methods
CSF proteomes were analyzed by quantitative Mass-Spectrometry. Spectra were identified using the Swissprot human and TIGR4 pneumococcal protein libraries. Proteins were quantitated and analyzed against mortality. Unique proteins in PM were identified against published normal CSF proteome. Random-Forest models were used to test for protein signatures discriminating outcome. Proteins of interest were tested for their effects on growth and neutrophil opsonophagocytic killing of
Results
CSF proteomes were available for 57 Adults with PM (median age 32 years, 60% male, 70% HIV-1 co-infected, mortality 63%). Three hundred sixty individual human and 23 pneumococcal proteins were identified. Of the human protein hits, 30% were not expressed in normal CSF, and these were strongly associated with inflammation and primarily related to neutrophil activity. No human protein signature predicted outcome. However, expression of the essential
Conclusions
Excessive
Identifiants
pubmed: 33363056
doi: 10.3389/fcimb.2020.603623
pmc: PMC7759504
doi:
Substances chimiques
Peptide Elongation Factor Tu
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
603623Subventions
Organisme : Medical Research Council
ID : MR/K012053/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/T016329/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 089671/B/09/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 101113/Z/13/Z
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R00/1871/1
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Wall, Brownridge, Laing, Terra, Mlozowa, Denis, Nyirenda, Allain, Ramos-Sevillano, Carrol, Collins, Gordon, Lalloo, Wren, Beynon, Heyderman and Brown.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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